VASOACTIVE HORMONES AND BLOOD PRESSURE REGULATION

这是一项建议， 肾功能的调节和区域 激肽释放酶-激肽和前列腺素系统，相互作用的激素 有助于调节肾功能的系统， 血压. 我们建议在以下领域进行试验。 领域1：机制和生理学研究 激肽对肾脏的调节作用及其意义 功能 实验将在灌注的肾脏中进行， 分离的肾小球和肾小管（a）以表征肾脏 激肽的作用与受体的关系 对cGMF和前列腺素合成（B）的影响 纯化激肽原对肾功能和肾内生成的影响 的kinins。 前列腺素和cGMP;和（c）评估肾功能 对激肽受体拮抗剂治疗的功能反应 过量的加压素或高蛋白质摄入， 会增加肾激肽 领域2：Kinins对血液贡献的研究 肾动脉开放对肾动脉高压的降压作用 高血压 实验将进行（a）调查 肾动脉扩张反应与肾动脉狭窄的关系 解夹和血浆水平和肾脏分泌的变化 激肽，和（B）评估激肽受体拮抗剂对 松开夹子时的痉挛反应 领域3：Kinins之间关系的研究， 肾组织前列腺素合成与甘油三酯代谢 髓细胞。 实验将在培养的家兔中进行 肾髓质间质细胞（a），以表征 研究激肽对RMIC甘油三酯脂肪酶的调节作用， cAMP介导的肾1激素和渗透压;和（B）检查 激肽和其他刺激甘油三酯的激素 脂肪酶活性对肾髓质合成类花生酸的影响 间质细胞

This is a proposal to continue characterizing the mechanisms of regulation and areas of functional responsibility of the renal kallikrein-kinin and prostaglandin systems, interacting hormonal systems which contribute to the regulation of renal function and blood pressure. We propose experimentation in the following areas. Area 1: Investigation of the Mechanisms and Physiological Significance of the Regulatory Influence of Kinins on Renal Function. Experiments will be conducted in perfused kidneys and isolated glomeruli and tubules (a) to characterize the renal actions of kinins in terms of receptors involved and relationship to cGMF and prostaglandin synthesis (b) to investigate the effects of purified kininogen on renal function and intrarenal generation of kinins. Prostaglandins and cGMP; and (c) to assess the renal functional response to treatment with kinin receptor antagonists with excess vasopressin or high protein intake, which conditions are known to increase renal kinins. Area 2: Investigation of the Contribution of Kinins to the Blood Pressure Lowering Effect of Renal Artery Unclipping in Renal Hypertension. Experiments will be conducted (a) to investigate relationships between the hypotensive response to renal artery unclipping and changes in plasma levels and renal secretion of Kinins, and (b) assess the effect of Kinin receptor antagonists on the hypotensive response to unclipping. Area 3: Investigation of the Relationship Between Kinins, Prostaglandin Synthesis and Trizlvceride Metabolism in Renal Medullary Cells. Experiments will be conducted in cultured rabbit renomedullary interstitial cells (a) to characterize the triglyceride lipase of RMIC and study its regulation by kinins, cAMP-mediated renal 1 hormones and osmolality; and (b) to examine the effects of kinins and other hormone that stimulate triglyceride lipase activity on eicosanoid synthesis by renomedullary interstitial cells.