NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS

这些研究人员提议设计和合成一系列 含负电官能团的双功能有机试剂 特定分子系绳长度的组和范围。这些 双官能团有机试剂用于化学修饰无细胞 用作载氧血液替代品的人血红蛋白 紧急输血。这些人造氧气载体将是免费的 艾滋病毒和其他通过输血传播的传染病病原体。这个 调查人员将验证分子结构配置， 新合成试剂的构象和系链长度，然后 探索试剂与天然氨基形成共价键的可能性 水介质中的酸性赖氨酸、半胱氨酸、缬氨酸。修饰后的血红蛋白 会被提纯，它们的物理组成和功能性质 将会被调查。

These investigators propose to design and synthesize a series of bifunctional organic reagents which contain negatively charged functional groups and range of specific molecular tether lengths. These bifunctional organic reagents are for chemically modifying cell-free human hemoglobins for use as oxygen carrying blood substitutes in emergency transfusions. These artificial oxygen carriers would be free of HIV and other transfusion-transmitted infectious agents. The investigators will verify molecular structures configuration, conformation and tether length of the newly synthesized reagents and then explore the reagents' potential to form covalent bonds with natural amino acids lysine cysteine valine in aqueous media. The modified hemoglobins will be purified and their physical composition and functional properties will be investigated.