DUFFY BLOOD GROUP ANTIGEN

描述：克隆了达菲蛋白的基因后， 研究人员建议：1。确定氨基的拓扑结构 通过免疫化学结合或双标记， 免疫电子显微镜（一种直观上不明显的方法; 2.确定抗体和疟疾的结合位点， 分子的外部部分; 3.确定非- 红系Duffy蛋白; 4.通过免疫化学确定哪些细胞 产生达菲蛋白; 5.确定化学因子结合要求; 6.找到并克隆小鼠等价基因; 7.研究 Duffy和Rh的相互作用，因为Duffy抗原（Fy 5）依赖于 在Rh蛋白的存在下进行表达。 长期 目标包括：1.函数的确定 糖蛋白; 2.确定达菲的重要性 就像大脑中的蛋白质一样; 3.确定结合到 疟疾生物体的裂殖子; 4.确定达菲是否 抗原是转运蛋白;和5.设计小鼠敲除模型， 确定达菲蛋白的重要性

DESCRIPTION: Having cloned the gene for the Duffy protein, the Investigators propose to: 1. determine the topology of the amino and carboxyl ends by immunochemical binding or double labeling immunoelectromicroscopy (an approach that is not intuitively obvious; 2. determine the antibody and malarial binding sites on the external portion of the molecule; 3. determine the sequence of non- erythroid Duffy protein; 4. determine by immunochemistry which cells produce Duffy proteins; 5. determine the chemikine binding requirements; 6. find and clone the mouse equivalent gene; and 7. study the interactions of Duffy and Rh since a Duffy antigen (Fy5) depends upon the presence of Rh proteins for its expression. Long-term goals consist of the following: 1. determination of the function of the glycoprotein; 2. determine the significance of a Duffy like protein in the brain; 3. determine the sequence the binds to the merozoites of malaria organisms; 4. determine whether the Duffy antigen is a transporter; and 5. design mouse knock-out models to determine the significance of the Duffy protein.