GENES CONTROLLING LORDOSIS BEHAVIOR

描述：这是一个项目第九年的续签。目标是 这项研究的目的是了解基本工作电路 产生前凸行为。私家侦探之前的工作取得了成果 关于参与控制的神经回路的有价值的信息 前凸。目前的工作将把这一点扩展到对 控制这种行为反应的分子机制。私人侦探将 本研究的重点是催产素在调节脊柱前凸中的作用 反应是因为a)它是行为相关基因，b)催产素是 在大脑中显示为前凸回路一部分的区域中被发现，以及b) 这种肽(催产素)及其受体的基因都是已知的。 本质上有两个具体目标。第一个是检查 阻断催产素基因对脊柱前凸和TO的影响 描述这种效应的特征。第二个目标是做基本相同的事情 催产素受体基因。 PI有初步数据，这些数据支持这一理论基础和 所提议的实验的可行性。例如，PI已经表明， 催产素对前凸的促进作用位于 VMN，一个含有催产素受体的部位。他还展示了 催产素的兴奋作用是通过催产素介导的 感受器。将反义寡核苷酸用于催产素受体，PI具有 研究发现，当女性服用雌二醇时，前凸会受到阻碍(但不会 雌激素和黄体酮)。最后，为了证明反义 技术是可行的，他从他的实验室提供的数据表明 反义寡核苷酸S用于PR S减少前凸，且PRIR减少 VMN.

DESCRIPTION: This is a renewal of a project in its ninth year. The goal of this research is to understand the basic working circuit for producing lordosis behavior. The PI's previous work has yielded valuable information on the neural circuitry involved in controlling lordosis. The current work will extend this to an analysis of the molecular mechanisms controlling this behavioral response. The PI will focus this research on the role of oxytocin in mediating the lordosis response because a) it is a behaviorally relevant gene, b) oxytocin is found in brain areas shown to be part of the lordosis circuitry, and b) the genes for both the peptide (oxytocin) and its receptor are known. There are essentially two specific aims. The first is to examine the effectiveness of blocking the oxytocin gene on lordosis and to characterize this effect. The second aim is to do basically the same thing for the oxytocin receptor gene. The PI has preliminary data which provide support for the rationale and feasibility of the proposed experiments. The PI has shown, for example, that the facilitatory effect of oxytocin on lordosis is situated in the VMN, a site containing oxytocin receptors. He has also demonstrated that the excitatory action of oxytocin is mediated via oxytocin receptors. Using antisense oligo's for the oxytocin receptor the PI has found that lordosis is blocked when females are given estradiol (but not estrogen followed by progesterone). Finally, to show that the antisense technology works, he provides data from his laboratory showing that antisense oligo s for PR s reduce lordosis, and that PRir is reduced in VMN.