BIOLOGICAL HAZARDOUS WASTE MANAGEMENT

这是一个研究创新和新颖的生物降解的建议 可适用于难降解化学品或复杂 化学品的混合物。 该系统基于细胞外酶， 催化自由基反应，降解木质素，性质 最难对付的化学物质 木质素是一种不溶性的，非立体选择性的， 复合杂聚物 我们认为一个进化到退化的系统 木质素应该可用于生物降解各种各样的 柠檬酸人造化学品 最终目标是减少 环境污染物的数量和毒性。 该系统应 适用于水性和固体基质， 化学品的使用可能不像其他系统那样关键。 还 真菌应该有一个主要的竞争优势，如果系统是基于 对木质纤维素营养的影响。 我们建议使用一种白色腐菌， 从生物学上降低与环境污染物相关的风险， 危险废物场或危险废物流。 它是一个高度 综合而又跨学科的计划，其中各种专业领域 对所有人都感兴趣的关键问题产生了影响， 个别调查员。 该计划将与其他 测试TNT和五氯苯酚生物修复系统的项目 受HMX污染的土壤和水。 该计划将成为 并支持环境中心的毒理学学位课程 毒理学。 预计几个学生将寻求联合 毒理学/分子生物学学位。 该计划将涉及教师在 化学和生物化学，生物学和环境工程在犹他州 州立大学和宾夕法尼亚州立大学分子和细胞生物学 圣母大学化学和生物化学。 这项研究将 包括分子生物学、重组DNA技术、酶工程 酶学，生物降解研究，真菌竞争因子 生物反应器的设计和工程。

This is a proposal to investigate an innovative and novel biodegradation system that may be applicable to difficult-to-degrade chemicals or complex mixtures of chemicals. The system is based upon extracellular enzymes that catalyze free radical reactions that evolved to degrade lignin, natures most recalcitrant chemical. Lignin is an insoluble, non-stereoselective, complex heteropolymer. We propose that a system that evolved to degrade lignin should be useful for the biodegradation of a wide variety of very recalcitrant, man-made chemicals. The ultimate objective is to reduce the amount and toxicity of environmental pollutants. The system should be adapted to both aqueous and solid matrices and it may be that availability of the chemical may not be as critical as with other systems. Also, the fungus should have a major competitive advantage if the systems are based upon lignocellulose nutrients. We propose to use a white rot fungus to biologically reduce the risk associated with environmental pollutants in hazardous waste sites or hazardous waste streams. It is a highly integrated yet interdisciplinary program where various areas of expertise are brought to bear on critical subjects of interest to all of the individual investigators. The program will be coordinated with other projects to test the system for the bioremediation of TNT and PCP contaminated soil and HMX contaminated water. The program will be part of and support the Toxicology Degree Program of the Center for Environmental Toxicology. It is expected that several students will pursue a joint Toxicology/Molecular Biology degree. The program will involve faculty in Chemistry and Biochemistry, Biology and Environmental Engineering at Utah State University, and Molecular and Cell Biology at Pennsylvania State University and Chemistry and Biochemistry at Notre Dame. The research will include molecular biology, recombinant DNA technology, enzyme engineering, enzymology, biodegradation studies, factors involved in fungal competition and bioreactor design and engineering.