CHARACTERIZATION OF STRESSOR INDUCED IMMUNE ALTERATION

众所周知，有压力的生活事件会影响进程。 疾病的威胁。有许多报告表明， 感染性疾病、自身免疫性疾病和恶性肿瘤 经历压力事件的病人。然而，免疫学 应激源引起的改变及其调节因素 有压力的经历和疾病之间的关系 没有得到很好的刻画。《公约》的总体目标 研究是为了阐明这些免疫变化并分离出 中介因素。这项研究的第一个目的是用在 体外技术以确定：(1)淋巴细胞、NK细胞、 和附属细胞会受到短期接触一种 应激源；(2)产生免疫改变的持续时间 应激源；(3)血浆中的物质或因素 从单个核细胞衍生出来的是压力的来源- 诱导改变；(4)产生激素的组织是否 对变更负责。将进行这项研究 以Lewis大鼠为实验对象，电击 作为压力源。我们的初步结果表明，一个短期的- 长期接触电击会导致反应性降低。 脾和全血淋巴细胞对刀豆蛋白A的作用。 应激源持续暴露到中等水平 导致正常的脾细胞对ConA的反应性；然而， 脾细胞的反应性降低在长期低血压后复发。 学期应激源暴露。第二个目标是描述那些 暴露于一种新生物引起的免疫学变化 中长期压力源。特别考虑将 归于脾血辨证作用 随着应激源暴露的量增加，淋巴细胞。这个 最终目标是直接评估应激源的影响 通过进行活体研究来暴露免疫功能。 这些研究将评估暴露在短时间内的影响， 中长期应激源对免疫功能的影响 使用一种免疫模型。我们的计划是评估 绵羊红细胞免疫应答(即免疫应答 需要T辅助细胞相互作用)，聚乙烯吡咯烷酮(PVP，a B淋巴细胞直接刺激因子)和念珠菌(一种真菌 与许多免疫缺陷有关)。这个 这项研究的意义不仅在于 增强对应激诱导的免疫改变的认识 功能和相关的健康益处，但也在 为进一步研究提供明确的免疫学基础 研究压力的心理方面。

It is well known that stressful life events can influence the course of disease. There are a number of reports of a higher incidence of infectious disease, autoimmune disease, and malignancies in patients experiencing stressful events. However, the immunologic alterations induced by a stressor and the factors which mediate the relationship between stressful experiences and disease have not been well-characterized. The overall objective of the research is to elucidate those immunologic alterations and isolate the mediating factors. The first aim of the research is to use in vitro techniques to determine: (1) how lymphocytes, NK cells, and accessory cells are affected by a short-term exposure to a stressor; (2) the duration of the immunologic alteration produced by the stressor; (3) whether substances in the plasma or factors derived from mononuclear cells are responsible for the stress- induced alteration; (4) whether hormone producing tissues are responsible for the alteration. The research will be conducted using Lewis rats as the experimental subjects and electric shock as the stressor. Our preliminary results indicate that a short- term exposure to electric shock produces a decreased reactivity of spleen and whole blood lymphocytes to Concanavalin A. Continuation of stressor exposure to an intermediate level resulted in normal spleen cell reactivity to Con-A; however, the decreased reactivity of the spleen cells recurred following long- term stressor exposure. The second aim is to characterize those immunologic changes that result from exposure to an intermediate and long-term stressor. Special consideration will be given to the differential effects observed in spleen and blood lymphocytes with increasing amounts of stressor exposure. The final aim is to make direct assessments of the effect of stressor exposure on immune function by performing in vivo studies. These studies will evaluate the effect of exposure to a short-, intermediate-, and long-term stressor on immune function by using an immunization model. The plan is to assess the immunization response to sheep erythrocytes (that response requires T helper cell interaction), polyvinylpyroloidone (PVP, a direct stimulator of B lymphocytes), and candida (a fungus associated with a number of immunologic defects). The significance of the research is viewed not only in terms of enhanced knowledge of the stress-induced alterations in immune function and the related health benefits, but also in terms of providing a well-defined immunologic basis for additional studies examining the psychological aspects of stress.