ELECTRICAL MEASUREMENT OF CELL MOTILITY IN VITRO

前列腺癌是最常见的癌症之一 在男人身上。先前的研究表明，这些肿瘤细胞倾向于 转移与它们在组织培养中的运动性相关，然而， 还不可能基于此设计出实用的预后测定法 事实这项研究的目标是采用一种创新的细胞运动 称为ECIS（电细胞-基质阻抗）的测量 传感）作为测定的基础，以确定 前列腺肿瘤 小鼠Dunning亚系的I期研究 前列腺腺癌，证明了几个显着的相关性， ECIS数据和亚系的转移潜力II期将延长 这些测量用于表征人前列腺癌细胞系。从 这项研究，最有效的ECIS协议，以区分 将选择转移能力并应用于分析原发性 从前列腺肿瘤生长物建立的培养物。一种装置将 设计、制造和测试，以简化临床分析， 前列腺穿刺活检和ECIS的副产物的形成 测定。一种临床预后方法的商业机会 前列腺癌是明确的。 此外，这部作品的出版将 促进ECIS方法作为研究细胞的通用研究工具 能动性

Prostatic carcinoma constitutes one of the most prevalent forms of cancer in men. Previous research has shown the propensity of these tumor cells to metastasize is correlated with their motility in tissue culture, yet it has not been possible to devise practical prognostic assays based on this fact. The goal of this research is to employ an innovative cell motility measurement referred to as ECIS (electric cell-substrate impedance sensing) as the basis of an assay to determine the metastatic potential of prostate tumors. Phase I research with Dunning sublines of murine prostatic adenocarcinoma, demonstrated several significant correlations of ECIS data and metastatic potential of the sublines Phase II will extend these measurements to characterize human prostatic cancer cell lines. From this research, the most effective ECIS protocols to discriminate metastatic abilities will be selected and applied to analyze primary cultures established from prostate tumor outgrowths. A device will be designed, fabricated, and tested to simplify clinical assays by combining both formation of outgrowths from prostate needle biopsies and ECIS assays. The commercial opportunities of a clinical method for prognosis of prostate cancer are clear. In addition, publication of this work will promote the ECIS method as a general purpose research tool to study cell motility.