EMBRYONIC AND EARLY POSTNATAL WEAVER MICE

这项研究方案将分子遗传学和神经生物学相结合，并 细胞生物学来确定weaver基因在小脑中的作用 发展。定位在基因附近的小鼠微卫星标记 将被用来鉴定胚胎织女纯合子。这项技术 将用于检查织布工小脑的发育 胚胎之前的明显形态表现为突变体。 将使用原位和免疫细胞化学分析来回答这些问题 问题。此外，用胚胎编织者重组培养物 颗粒细胞将决定颗粒细胞中最早的时间 突变有影响的发展。拯救这些文化 具有野生型颗粒的细胞将决定小脑的时间 织布者配基存在的发展。

This research proposal combines molecular genetics with neurobiology and cell biology to define the role of the weaver gene in cerebellar development. Mouse microsatellite markers that map close to the gene will be used to identify embryonic weaver homozygotes. This technology will be used to examine the development of the cerebellum in weaver embryos prior to the apparent morphological manifestations of the mutant. In situ and immunocytochemistry analysis will be used to answer these questions. In addition, reaggregate cultures with embryonic weaver granule cells will determine the earliest time in granule cell development that the mutation has an effect. Rescue of these cultures with wild type granule cells will determine the time in cerebellar development that the weaver ligand is present.