HISTOPATHOLOGY, CBF AND NMR ASSESSMENT OF STROKE

本申请旨在调查 水和局灶性脑缺血的生物物理环境， 核磁共振（NMR）。 减少的程度和持续时间 局部脑血流量（rCBF）和明确的生物学终点- 组织损伤点将根据NMR可测量的 在中风（大脑中动脉闭塞）的大鼠模型中的参数。 水的1H NMR可测量参数（T1、T2、p和ADCw）为 对水的生物物理环境的变化敏感（即， 旋转、平移、集中）。 核磁共振成像将用于 描述变化的空间分布和时间演变 在水的生物物理环境中。 此外，一种用于 缺血诱导的表观扩散系数的降低 水（ADCw）将被[调查]。 有人建议， 了解水的生物物理环境的变化将 导致更好地理解缺血性心脏病的机制， 脑损伤 这种方法最终可能会为非- 对中风的程度、严重程度和状态的侵入性测定 导致人类脑损伤

This application seeks to investigate relationships between changes in the biophysical environment of water and focal cerebral ischemia using Nuclear Magnetic Resonance (NMR). The degree and duration of reductions in regional cerebral blood flow (rCBF) and a well defined biological end- point of tissue damage will be characterized in terms of NMR-measurable parameters in a rat model of stroke (middle cerebral artery occlusion). The 1H NMR-measurable parameters of water (T1, T2, p and ADCw), are sensitive to changes in the biophysical environment of water (i.e., rotation, translation, concentration). NMR imaging will be used to characterize the spatial distribution and temporal evolution of changes in the biophysical environment of water. Additionally, a mechanism for the ischemia-induced reduction in the apparent diffusion coefficient of water (ADCw) will be [investigated]. It is proposed that an understanding of changes in the biophysical environment of water will lead to a better understanding of the mechanisms involved in ischemic brain damage. This approach may eventually provide a means for the non- invasive determination of the degree, severity, and status of stroke induced brain damage in humans.