SYNAPTIC VESICLE MEMBRANE PROTEINS

胞吐作用释放激素和神经递质的过程 涉及钙刺激的附着和分泌或融合 内分泌细胞或神经元质膜上的突触小泡。 这一过程可能是由特定的蛋白质成分引导的。 分泌或突触小泡、细胞质和质膜。 这个项目的目标是确定具体的重要性 胞吐作用中的囊泡膜蛋白及其生化细节 它们的作用机制。这项研究最初将重点放在 具有细胞质结构域的跨膜蛋白--突触素 与蛋白激酶C的钙和脂结合结构域同源， 以及突触素，另一种跨膜蛋白，具有延长的， 可与细胞质或质膜相互作用的富含脯氨酸结构域 组件。将采用一种新的方法来表达 酵母中哺乳动物对这些蛋白的cDNA，生化特征 酵母分泌囊泡的特性 蛋白质，并检查分泌物的生理学 转化的酵母细胞。哺乳动物的结构特征 对生化或生理作用至关重要的蛋白质将是 由定点突变确定。对中国的基本认识 激素释放和神经传递的机制研究进展 这项研究可能会导致对精神障碍的治疗的发展 神经肌肉传递、中枢神经系统功能或内分泌 功能。

The process of hormone and neurotransmitter release by exocytosis involves the calcium-stimulated attachment and fusion of secretory or synaptic vesicles to the plasma membrane of endocrine cells or neurons. This process is presumably directed by specific protein components of the secretory or synaptic vesicle, the cytoplasm, and the plasma membrane. The goal of this project is to determine the importance of specific vesicle membrane proteins in exocytosis and the biochemical details of their mechanism of action. The study will initially focus on synaptotagmin, a transmembrane protein that has a cytoplasmic domain homologous to the calcium and lipid-binding domain of protein kinase C, and synaptophysin, another transmembrane protein that has an extended, proline rich domain that may interact with cytoplasmic or plasma membrane components. A novel approach will be adopted involving the expression of mammalian cDNAs for these proteins in yeast, biochemical characterization of the properties of yeast secretory vesicles conferred by these proteins, and examination of the physiology of secretion in the transformed yeast cells. The structural features of the mammalian proteins essential for biochemical or physiological effects will be determined by site-directed mutagenesis. The basic understanding of the mechanisms underlying hormone release and neurotransmission gained in this study may lead to the development of treatments for disorders of neuromuscular transmission, central nervous system function, or endocrine function.