GONADOTROPIN RECEPTORS

我的职业目标是在一个 这将促进我的研究项目的发展。 很明显 爱荷华州大学生理学和生物物理学系提供 这样的环境。 我的研究主要集中在LH/CG和FSH 受体，其功能在生殖生理学中起着关键作用。 我们希望逐步更详细地了解这些结构， 受体及其作用机制的基础机制， 调控 我们最近的能力大大推进了这些研究 克隆大鼠LH/CG和FSH受体的cDNA。 因此，我们现在有 解决重要生理问题的实验工具 关于这些受体的生物学。 具体而言，总体 我为我的实验室设定的未来五年的具体目标是： （1）检查卵巢和睾丸的诱导和/或调节 转录和翻译水平的LH/CG和FSH受体;（2） 克隆、表达和表征LH/CG的5'侧翼区， FSH受体基因，以研究细胞特异性和 这些受体的神经调节表达;和（3）映射功能性 结构域的LH/CG和FSH受体，通过构建和表征 受体的突变体和嵌合体形式。 从这些研究中获得的信息将大大有助于我们 了解睾丸和卵巢功能的调节，以及 人类的生育能力和不育能力。 他们还将为我们的 了解G蛋白偶联受体 将细胞外事件转化为细胞内信号。 正在请求提供薪金支助，以减少我 我有义务花在教学和委员会的职能，让我 把更多的时间（至少80%）花在实验室上。 这将是 这对维持我正在进行的研究的势头至关重要，因此 进一步帮助我成为一名独立调查员

My career goals have been to attain a tenured position in an environment that will foster the growth of my research program. It is clear that the Department of Physiology and Biophysics at The University of Iowa offers such an environment. My research has focused upon the LH/CG and FSH receptors, whose functions play a pivotal role in reproductive physiology. We hope to learn in progressively greater detail the structure of these receptors and the mechanisms underlying their mechanism of action and regulation. These studies have been greatly advanced by our recent ability to clone the cDNA's for the rat LH/CG and FSH receptors. Thus, we now have the experimental tools to address important physiological questions concerning the biology of these receptors. Specifically, the overall specific aims that I have set for my laboratory for the next five years are to (1) examine the induction and/or regulation of ovarian and testicular LH/CG and FSH receptors at the levels of transcription and translation; (2) clone, express, and characterize the 5' flanking regions of the LH/CG and FSH receptor genes in order to study the cell-specific and hormonally-regulated expression of these receptors; and (3) map functional domains of the LH/CG and FSH receptors by constructing and characterizing mutant and chimeric forms of the receptors. The information gained from these studies will significantly aid in our understanding of the regulation of testicular and ovarian function, as well as human fertility and infertility. They will also contribute to our understanding of the mechanisms by which G protein-coupled receptors transmit extracellular events into intracellular signals. Salary support is being requested in order to reduce the amount of time I am obligated to spend in teaching and committee functions, allowing me to devote more of my time (at least 80%) to the laboratory. This will be critical for sustaining the momentum of my ongoing research, and thus further help establish myself as an independent investigator.