PROKARYOTIC GENE REGULATION BY LIGHT AND OXYGEN

申请人的实验室最近确定了五种反式作用 调控原核基因表达的调控因子 氧张力和光强度的变化。 在拟议 在融资期间，这些调节因素中的每一个都将是遗传性的， 以其在控制基因表达中的作用为生物化学特征 对氧气和光线的反应两个"氧反应"调节 蛋白质是双组分反应家族的额外成员 这表明一个调节蛋白是一个膜 跨越传感器激酶，通过以下途径传递有关厌氧的信息 DNA结合反应调节剂的磷酸化。 附加传感器 描述了一种激酶，其被认为与光的感知有关 通过监测电子传递链的氧化还原状态来确定强度。 该另外的氧化还原响应调节剂在结构上类似于一种氧化还原响应调节剂。 线粒体中的蛋白质，已知其参与 细胞色素氧化酶;一种与退行性疾病有关的蛋白质， 帕金森氏症和缺血性心脏病。该实验室还 表征参与控制的光敏感调节蛋白， S-腺苷高半胱氨酸水解酶（SAHase）的表达。 SAHase是细胞毒性作用的重要促成因素， 腺苷导致腺苷患者严重免疫缺陷 脱氨酶（ADA）缺乏。

The applicants laboratory has recently identified five trans-acting regulatory factors governing prokaryotic gene expression in response to changes in oxygen tension and light intensity. During the proposed funding period, each of these regulatory factors will be genetically and biochemically characterized for their role in controlling gene expression in response to oxygen and light. Two "oxygen responding" regulatory proteins are additional members of a family of two-component response regulators which indicates that one regulatory protein is a membrane spanning sensor kinase that transmits information about anaerobioses via phosphorylation of a DNA binding response regulator. An additional sensor kinase is described which is thought to be involved in sensing light intensity by monitoring the redox state of the electron transport chain. This additional redox responding regulator is structurally similar to a protein in mitochondria which is known to be involved in expression of cytochrome oxidase; a protein implicated in degenerative diseases such as Parkinson's and Ischemic heart disease. The laboratory is also characterizing a light sensing regulatory protein involved in controlling expression of the enzyme S-adenosyl homocysteine hydrolase (SAHase). SAHase is an important contributing factor in the cytotoxic effects of adenosine leading to severe immune deficiency in patents with adenosine deaminase (ADA) deficiency.