Genetic Analysis of Mg-tetrapyrrole Biosynthesis

四吡咯镁生物合成的遗传分析

• 批准号: 6877141
• 负责人: CARL Eugene BAUER
• 金额: $ 24万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877141
• 关键词: Rhodospirillales; bacterial genetics; biosynthesis; carotenoids; chlorophyll; enzyme activity; gene induction /repression; heme; nitrogenase; oxidation reduction reaction; oxidoreductase; photosynthesis; tetrapyrroles; transcription factor

DESCRIPTION (provided by applicant): The tetrapyrrole biosynthetic pathway is responsible for synthesizing important metabolities such as vitamin B12, hemes, bilins and chlorophylls. The "common trunk" of the pathway, from 5-aminolevulinate to protoporphyrin IX, has received much attention owing to the fact that a number of heredity diseases (porphyrias) are caused by the overproduction of heme precursors. Clinical manifestations of overproducing these intermediates range from simple skin lesions, to psychotic disorders, to death. The vitamin B12 branch of the pathway has also received recent attention genes involved in vitamin B12 synthesis characterized from Pseudomonas denitrificans and in Salmonella typhimurium. In contrast to the wealth of information on heme and vitamin B12 synthesis, information is just emerging on the synthesis of the Mg-tetrapyrrole family of chlorophylls. In this proposal, we outline plans to perform detailed biochemical and genetic analysis of the Mg-tetrapyrrole biosynthetic pathway. This analysis includes (i) biochemical characterization of enzymes from the Mg-tetrapyrrole branch of the biosynthetic pathway, (ii) biochemical and genetic characterization of a redox responding transcription factor that regulates expression of heme, Mg-tetrapyrroles and carotenoid biosynthesis genes, as well as polypeptides that comprise the light harvesting-II portion of the photosystem. A thorough understanding of the tetrapyrrole biosynthetic pathway has some far ranging practical implications, such as the design of herbicides that target enzymes in the Mg tetrapyrrole pathway, and the health implications of overproducing tetrapyrrole end-products such as vitamin B12 and heme. It should also not be overlooked that tetrapyrrole driven photosynthesis is the primary route of capturing and supplying energy to living cells and, consequently, it is the most important source of energy in our technological world.

描述（由申请人提供）：四吡咯生物合成途径是 负责合成重要的代谢物，如维生素B12，血红素， 胆色素和叶绿素。路径的“共同主干”，从 5-氨基乙酰丙酸到原卟啉IX，受到了很大的关注， 事实上，许多遗传性疾病（卟啉症）是由 血红素前体的过量产生。过度生产的临床表现 这些中间疾病的范围从简单的皮肤损伤到精神障碍， 死亡该途径的维生素B12分支最近也受到关注 假单胞菌中参与维生素B12合成的基因 沙门氏菌和鼠伤寒沙门氏菌。 与血红素和维生素B12合成的丰富信息相反， 信息是刚刚出现在镁-四吡咯家族的合成， 叶绿素在这份提案中，我们概述了执行详细计划的计划， 镁-四吡咯生物合成途径的生化和遗传分析。 该分析包括（i）来自该菌株的酶的生物化学表征。 镁-四吡咯分支的生物合成途径，（ii）生物化学和 氧化还原应答转录因子的遗传表征， 调节血红素、镁-四吡咯和类胡萝卜素生物合成的表达 基因，以及包含光收获-II部分的多肽， 光系统。 对四吡咯生物合成途径的深入了解， 广泛的实际影响，如除草剂的设计， 镁四吡咯途径中的酶，以及 过量产生四吡咯终产物如维生素B12和血红素。应当 同样不可忽视的是，四吡咯驱动的光合作用是主要的 捕获和提供能量给活细胞的途径，因此， 是我们科技世界中最重要的能源

项目成果

ALTERED MONOVINYL AND DIVINYL PROTOCHLOROPHYLLIDE POOLS IN BCHJ MUTANTS OF RHODOBACTER-CAPSULATUS - POSSIBLE MONOVINYL SUBSTRATE DISCRIMINATION OF LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE

• DOI: 10.1074/jbc.270.8.3732
• 发表时间: 1995-02-24
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: SUZUKI, JY;BAUER, CE
• 通讯作者: BAUER, CE

Light-independent chlorophyll biosynthesis: involvement of the chloroplast gene chlL (frxC).

不依赖光的叶绿素生物合成：叶绿体基因 chlL (frxC) 的参与。

• DOI: 10.1105/tpc.4.8.929
• 发表时间: 1992
• 期刊: The Plant cell
• 作者: Suzuki,JY;Bauer,CE
• 通讯作者: Bauer,CE

Genetic analyses of photopigment biosynthesis in eubacteria: a guiding light for algae and plants.

真细菌感光色素生物合成的遗传分析：藻类和植物的指路明灯。

• DOI: 10.1128/jb.175.13.3919-3925.1993
• 发表时间: 1993
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Bauer,CE;Bollivar,DW;Suzuki,JY
• 通讯作者: Suzuki,JY

Nucleotide Sequence of S-Adenosyl-l-Methionine: Magnesium Protoporphyrin Methyltransferase from Rhodobacter capsulatus.

S-腺苷-l-甲硫氨酸的核苷酸序列：来自荚膜红杆菌的镁原卟啉甲基转移酶。

• DOI: 10.1104/pp.98.1.408
• 发表时间: 1992
• 期刊: Plant physiology
• 影响因子: 7.4
• 作者: Bollivar,DW;Bauer,CE
• 通讯作者: Bauer,CE

A prokaryotic origin for light-dependent chlorophyll biosynthesis of plants.

植物光依赖性叶绿素生物合成的原核起源。

• DOI: 10.1073/pnas.92.9.3749
• 发表时间: 1995
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Suzuki,JY;Bauer,CE
• 通讯作者: Bauer,CE