CORE--ADVANCED TECHNOLOGY LABORATORIES

ACTU病毒学核心实验室已经并将继续发挥作用 在评价实验性抗病毒治疗中的重要作用 ACTG人群中HIV-1感染情况。有效性研究的发展与评价 HIV-1感染的治疗受到较长时间间隔的限制 临床终结点。此外，血清阳性婴儿的入学人数 母亲进入临床试验受到诊断难度的限制 HIV-1感染源于胎盘转移母体的HIV-1抗体。 因此，需要病毒学分析的可用性来1)评估 抗病毒药物治疗对病毒载量和病毒耐药性的影响 2)诊断血清阳性婴儿中的HIV-1感染。 拟议的研究有以下具体目标： 1.为ACTG议定书规定的区域实验室提供支持 包括人类免疫缺陷病毒1型培养在内的病毒学检测 (HIV-1)和从临床样本中检测HIV-1 p24抗原水平。 2.评估新的临床相关的HIV-1病毒学标志物 在ACTG协议中实现。 Case Western Reserve University(CWRU)ACTU病毒学实验室 经认证可提供HIV-1培养测试和血清HIV-1测量 ACTG方案的P24抗原水平。该实验室有能力 进行多达1000种HIV-1培养和5000种血清HIV-1 p24抗原水平 每年。此外，该实验室目前正在进行定量检测 血浆和细胞培养，定性和定量 非同位素聚合酶链式反应(PCR)和血清p24抗原 酸处理后进行检测。CWRU ACTU病毒学实验室拥有 成为ACTG区域病毒学核心的能力和专业知识 实验室。

The ACTU Virology Core laboratory has and will continue to play an essential role in the evaluation of experimental antiviral treatment for HIV-1 infection in the ACTG. The development and evaluation of effective therapies for HIV-1 infection is limited by the long time interval to clinical endpoints. In addition, enrollment of infants of seropositive mothers into clinical trials is limited by the difficulty in diagnosing HIV-1 infection due to placental transfer of maternal HIV-1 antibody. Therefore, the availability of virological assays is needed to 1) assess the effect of antiviral drug therapies on viral load and viral resistance and 2) diagnose HIV-1 infection in seropositive infants. The proposed study has the following specific aims: 1. To provide regional laboratory support for ACTG protocol-mandated virology testing including culture of Human Immunodeficiency Virus Type 1 (HIV-1) and measurement of HIV-1 p24 antigen levels from clinical samples. 2. To evaluate new clinically relevant HIV-1 virological markers for implementation in ACTG protocols. The Case Western Reserve University (CWRU) ACTU Virology laboratory is certified to provide HIV-1 culture testing and measurement of serum HIV-1 p24 antigen levels for ACTG protocols. The laboratory has the capacity to perform up to 1000 HIV-1 cultures and 5000 serum HIV-1 p24 antigen levels per year. In addition, the laboratory is currently performing quantitative HIV-1 plasma and cell cultures, qualitative and quantitative HIV-1 nonisotopic polymerase chain reaction (PCR) assays, and serum p24 antigen assays after acid treatment. The CWRU ACTU Virology laboratory has the capacity and expertise to become and ACTG regional virology core laboratory.