DROSOPHILA TRNA 5' AND 3' END PROCESSING

描述（改编自申请人摘要）：研究者 建议研究细胞中tRNA的5'和3'加工的要求 黑腹果蝇 RNase P，5'端所需 处理，3 '-tRNase将被分离和表征， 大分子组成 由于RNase P通常具有RNA 任何与果蝇RNase P共纯化的RNA都将被测序， 为了进行系统发育比较与RNA相关的 其他物种的RNase P。 将位点特异性突变引入tRNA以分析 对底物识别和催化的要求。 三个问题 将在这些研究中得到解决：1。tRNA的成分是如何 RNase P和3 '-tRNase识别的结构是否不同？ 2.）的情况。这两种酶是识别特定的序列，还是只识别结构？ 和3.）三级接触（例如，在D和 T环）有助于底物识别？

DESCRIPTION (Adapted from the applicant's abstract): The investigator proposes to study requirement for 5' and 3' processing of tRNA in cells of Drosophila melanogaster. Both RNase P, required for 5' end processing, and 3'-tRNase will be isolated and characterized as to their macromolecular composition. Since RNase P generally has an RNA component, any RNA copurifying with Drosophila RNase P will be sequenced in order to make phylogenetic comparisons with RNAs associated with RNase P in other species. Site specific mutations will be introduced into tRNA to analyze requirements for substrate recognition and catalysis. Three questions will be addressed in these studies: 1.) How do the elements of tRNA structure recognized by RNase P and 3'-tRNase differ from each other? 2.) Does either enzyme recognize a specific sequence, or only structure? and 3.) To what extent do tertiary contacts (e.g., between the D and T loops) contribute to substrate recognition?