XENOTRANSPLANTATION EVALUATION--HUMAN TISSUE/NUDE MICE

实验总结：为了提供免疫缺陷的胸腺裸鼠， 足够的设施和专业知识，用于人体组织的长期研究 异种移植物作为研究人类的生物测定系统 致癌作用 概述：提供一只自我维持的裸鼠 用于新鲜人体组织和/或体外长期研究的菌落 在人细胞中诱导转化用于体内表达， 在小鼠中移植恶性肿瘤用于癌症中断研究。 具体而言：提供常规能力（1）12个月生存期 人体组织和细胞皮下移植的研究， 具有活的上皮和正常组织学的体内植入物，在自体- 持续的，屏障菌落只能由抗菌人员进入 淋浴和高压灭菌器的供应;（2）长期生存研究 人器官组织异种移植物;（3）长期生长、增殖和 用于体内表达的细胞的致瘤性研究 实验诱导的肿瘤前和肿瘤转化 选择化学试剂和基因转染。材料提取 组织学切片制备和诊断， 维护和扩展NCI开发的dBASE-IBM PC集群数据 饲养员和实验动物的兽医保健 动物必须支持10的持续测定要求 科学家平均发起50个新实验，使用2,000多个 小鼠/年。

SUMMARY OF OBJECTIVES: To provide immunodeficient, a thymic nude mice, adequate facilities, and expertise for long-term studies of human tissue xenotransplants as the biological assay system for studying human carcinogenesis. GENERALLY: Provide a self-sustaining, nude mouse colony for Long-term studies of fresh human tissues, and/or in vitro induced transformation in human cells for in vivo expression and transplantation of malignancies in mice for cancer interruption studies. SPECIFICALLY: Provide as routine capability (1) 12 month survival studies of human tissues and cells transplanted subcutaneously as in vivo implants with viable epithelium and normal histology, in a self- sustaining, barrier colony accessible only by antibacterial personal showers and autoclaves for supplies; (2) Long-term survival studies of human organ tissue xenografts; (3) Long-term growth, proliferation, and tumorigenicity studies of cells for in vivo expression of preneoplastic and neoplastic transformation experimentally induced by selected chemical agents and genetic transfections. Materials Pick-up & delivery, Histopathology slide preparations and diagnoses, maintenance and expansion of the NCI-developed, dBASE-IBM PC colony data base, and veterinarian health care for breeders and experimental animals must support the continuing assay requirement of 10 scientists initiating an average of 50 new experiments, using 2,000+ mice/year.