FREE RADICALS & AGED-INDUCED NEUROCHEMICAL CHANGES IN THE MONKEY

本研究的目的是确定氧衍生的 自由基在大脑老化中的作用 这一目标将通过以下方式实现： 获得年龄对自由的不同方面的影响的测量结果 形成和代谢的几个领域的猴子的大脑。 的参数 测定的指标包括脂质过氧化、超氧化物的活性 谷胱甘肽过氧化物酶和过氧化氢酶，游离 自由基清除剂，包括谷胱甘肽、抗坏血酸和尿酸，和 脑上清液抑制自由基形成的能力。 我们 清除剂测量方法，使用16 通道探测器系统，将使我们能够同时确定 浓度的单胺，它们的前体和代谢物在相同的 组织样本 关于选定国家的活动的补充资料 神经递质系统将通过测量胆碱 乙酰转移酶活性和GABA浓度。 关系 将探讨自由基代谢和神经递质水平。 在 此外，本项目中测量的所有变量将与 行为、生物化学和形态学与年龄相关的变化， 同样的猴子

The goal of this investigation is to determine the role of oxygen-derived free radicals in aging of the brain. This goal will be approached by obtaining measurements of the effect of age on different aspects of free formation and metabolism in several areas of monkey brain. The parameters to be measured include lipid peroxidation, activity of superoxide dismutase, glutathione peroxidation, and catalase, concentrations of free radical scavengers including glutathione, ascorbic acid and uric acid, and the ability of brain supernatant to inhibit free radical formation. Our method of scavenger measurement, HPLC with coulometric detection using a 16 channel detector system, will allow us to determine simultaneously concentrations of monoamines, their precursors and metabolites in the same tissue sample. Additional information on the activity of selected neurotransmitter systems will be obtained by the measurement of choline acetyltransferase activity and GABA concentrations. Relationship between free radical metabolism and neurotransmitter levels will be explored. In addition, all variables measured in this project will be correlated with behavioral, biochemical and morphological age-related changes found in the same monkeys.