GENETIC DETERMINANTS OF HIGH BP IN THREE RACIAL GROUPS

该网络提出了五个具体目标，以实现本地化和 描述高BP的遗传决定因素。目标1将使用健壮 500对高血压同胞中的同胞对连锁分析 种族组(总共1500对兄弟姐妹)定位影响基因 EHYT发生的个体差异。目标2和目标3将 利用之前收集的BP和中间预测因子特性 来自罗切斯特(MN)家庭心脏研究的1488对兄弟姐妹数据 定位导致BP和EHYT的基因。拟议的连锁分析 (目标1-3)将使用广泛的候选基因阵列和大型 整个基因组中的匿名标记的数量。AIM 4将使用多个 连锁基因的双等位基因序列多态和分支分析 基因以确定单倍型，以便进一步进行DNA测序 确定导致以下因素的候选功能性DNA序列变异 血压水平和EHYT状态的个体间差异。目标5将 评估候选功能性DNA序列变异的能力 预测EHYT在三个种族群体中的地位。

This Network proposes to carry out five specific aims to localize and characterize the genetic determinant of high BP. Aim 1 will use robust sibling pair linkage methods in 500 hypertensive sibling pairs in each racial group (a total of 1500 sibling pairs) to localize genes influencing interindividual differences in the occurrence of EHYT. Aims 2 and 3 will take advantage of previously collected BP and intermediate predictor trait data rom 1488 sibling pairs from the Rochester (MN) Family Heart Study to localize genes contributing to BP and EHYT. The proposed linkage analyses (Aims 1-3) will use both an extensive array of candidate genes and a large number of anonymous markers throughout the genome. Aim 4 will use multiple diallelic sequence polymorphisms and cladistic analyses within a linked gene to identify haplotypes for further DNA sequencing in order to identify candidate functional DNA sequence variation contributing to interindividual differences in BP level and EHYT status. Aim 5 will evaluate the ability of candidate functional DNA sequence variation to predict EHYT status in the three racial groups.