INTERNATIONAL WORKSHOP ON HUMAN Y CHROMOSOME

人类Y染色体是人类基因组中最小的染色体， 含有约30Mb的常染色质。因为它的遗传简单性， 实际信息性序列可以小到10-14Mb，并且已经 估计含有大约200-350个功能基因。大多数这些基因 要么与男性生理有关，要么是多基因性状的成员。 首届人类Y染色体定位国际研讨会召开 于1994年4月2日至5日在英国剑桥休斯音乐厅举行。这 来自8个国家的科学家参加了研讨会，其中包括15个国家 来自美国的参与者。已经实现了许多目标 通过剑桥研讨会，包括达成共识 整个常染色质的缺失和物理重叠群图谱 亚区，14个基因的定位和这些图谱上的许多新的STS， X-Y同源性的定义，人和小鼠Y的比较图谱 EST的染色体和定位克隆策略的评价 从这条染色体上收集。尽管取得了这些进展，但还有更多的工作要做 需要达到确定完整基因的最终目标 这条染色体的信息。首先，许多区域的删除和 YAC、PI克隆和宇宙中的物理重叠群图必须是 结合到整体物理重叠群图中，从而构建了 常染色质的高分辨率物理地图。这一点从 对大规模位置克隆技术的巨大兴趣， 在cDNA和捕获的外显子方面的其他EST正在被分离 从许多实验室的这条染色体中分离出来。这些EST将需要 整合到人类Y基因的第一代转录图谱中 染色体。 基于上述发展，我们建议进行第二次 人类Y染色体国际研讨会九月十七日举行-- 1995年，在加利福尼亚州太平洋格罗夫的阿西洛玛会议中心。这 研讨会将重点建设共识删除和实体 整个常染色质及其亚区的重叠群图谱 X-Y同调和比较映射，以及第一个- 人类Y染色体的世代转录图谱。这将是一场 对这一领域的调查人员来说是一个重要的机会 展示他们的结果，协调并讨论他们之间的差异 发现，并在研究中相互交流和合作 共同的利益。拟议中的研讨会将是一个重要的里程碑 朝着彻底确定基因的最终目标迈进 人类Y染色体上的内容。

The human Y chromosome is the smallest chromosome in the human genome and contains about 30 Mb of euchromatin. Because of its genetic simplicity, the actual informative sequence may be as little as 10-14 Mb and has been estimated to contain about 200-350 functional genes. Most of these genes are either related to male physiology or members of polygenic traits. Recently the First International Workshop on Human Y Chromosome Mapping was held on April 2-5,1994 at Hughes Hall, Cambridge, England. This workshop was attended by 64 scientists from 8 countries, including 15 participants from the United States. Numerous goals have been achieved through the Cambridge Workshop, including the generation of consensus deletion and physical contig maps of the entire euchromatin and subregions, localization of 14 genes and many new STSs on these maps, definition of X-Y homology, comparative mapping of human and mouse Y chromosomes and evaluation of positional cloning strategies for EST collection from this chromosome. Despite these advances, more work is needed to achieve the ultimate goal of determining the complete genetic information of this chromosome. First, many of the regional deletion and physical contig maps in YACs, PI clones and cosmids have to be incorporated into the overall physical contig map, thereby constructing a higher resolution physical map of the euchromatin. As evidenced by the tremendous interests in large-scale positional cloning techniques, additional ESTs in terms of cDNAs and trapped exons are being isolated from this chromosome in many laboratories. These ESTs will needed to be incorporated into a first generation transcription map of the human Y chromosome. Based on the above developments, we propose to conduct a Second International Workshop on Human Y Chromosome to be held on September 17- 20,1995 at the Asilomar Conference Center, Pacific Grove, California. This workshop will focus on the construction of consensus deletion and physical contig maps of the entire euchromatin and the subregions, on advances of X-Y homology and comparative mappings, and the assembly of a first- generation transcription map of the human Y chromosome. It will be an important opportunity for investigators in this field to assemble and present their results, reconcile and discuss about differences in their findings, and interact and collaborate with each others in studies of mutual interests. The proposed workshop will be an important landmark towards the ultimate goal of a complete determination of the genetic content on the human Y chromosome.