CELL DIVISION GENE PRODUCTS OF BACILLUS SUBTILIS

描述：细菌细胞分裂是一个复杂的、高度调控的过程。 其中细胞被膜组分的生物合成被重新定向以形成 分区隔膜。这一过程在时间和空间上都受到调控， 以确保蜂窝组件的均匀分区，包括 细菌染色体。隔膜的形成必须涉及协调合成 细胞壁和细胞膜成分的细胞膜。这个 细菌细胞分离部位的化学性质仍然是最常见的 原核生物生物学中仍有重大悬而未决的问题。这个 细菌细胞分裂装置的高度保守性使这 工艺是未来抗菌药物发展的可能目标 化疗药物。 该项目涉及两个微型细胞的分子遗传学特征。 决定因素(Minc和divlVA)和三个细胞形状决定因素(MreBCD) 革兰氏阳性细菌枯草芽孢杆菌。这些人的行动地点 细菌细胞中的蛋白质将通过免疫荧光进行检测 技巧。微细胞基因编码的蛋白质是已知最早的 参与细菌细胞分裂过程的作用蛋白。 因此，它们的目标是新生的分隔点，而不是大部分 细胞分裂蛋白参与了这一过程。网站上的 参与细胞定位的微细胞相关蛋白 分离部位的蛋白质将被鉴定出来。DivlVA蛋白 已知参与该菌内孢子的形成过程 细菌，一个涉及不对称分离事件的过程。这个 DivlVA细胞分裂蛋白和细胞分裂蛋白之间的界面性质 将检查细菌产孢机。Mre细胞形状 决定基因编码的蛋白质通过影响细胞形态 它们参与了细胞分裂过程。MreC蛋白已经被 显示在指数生长的细胞中定位于分裂隔膜 枯草芽孢杆菌。MreB、MreC和MreD蛋白在骨肉瘤中的作用 将研究除法过程。通过对抑制者的隔离 突变，与mre蛋白相互作用的蛋白质将被鉴定。

DESCRIPTION: Bacterial cell division is a complex, highly regulated process in which biosynthesis of the cell envelope components is reoriented to form a division septum. The process is regulated both temporally and spatially, to ensure equal partitioning of cellular components including a copy of the bacterial chromosome. Septum formation must involve coordinated synthesis of cell wall and cell membrane components of the cell envelope. The chemical nature of the bacterial cell septation site remains one of the most significant unanswered questions remaining in procaryotic biology. The highly conserved nature of the bacterial cell division apparatus makes this process a likely target for the development of future antibacterial chemotherapeutic agents. The project involves a molecular genetic characterization of two minicell determinants (minC and divlVA) and three cell shape determinants (mreBCD) of the Gram-positive bacterium Bacillus subtilis. The sites of action of these proteins in the bacterial cell will be examined by immunofluorescence techniques. The minicell genes encode proteins which are the earliest known acting proteins involved in the process of bacterial cell division. Therefore, they target the nascent septation site before the bulk of the cell division proteins become involved in the process. Sites on the minicelling-associated proteins which are involved in the localization of the proteins to the septation site will be identified. The DivlVA protein is known to be involved in the process of endospore formation in this bacterium, a process which involves an asymmetric septation event. The nature of the interface between the DivlVA cell division protein and the bacterial sporulation machinery will be examined. The mre cell shape determination genes encode proteins which affect cell morphology through their involvement in the cell division process. The MreC protein has been shown to be localized at the division septa in exponentially growing cells of B. subtilis. The roles of the MreB, MreC, and MreD proteins in the division process will be studied. Through the isolation of suppressor mutations, proteins which interact with the Mre proteins will be identified.