Brillouin microscopy for high-speed imaging of rigidity within cells

本项目旨在提高布里渊显微镜的灵敏度和速度。布里渊显微镜使用光来测量样品的三维刚度，而不需要物理接触，允许它们在细胞内部或完整组织等不可接近的位置使用。然而，布里渊显微镜速度太慢，不能用于大多数研究。该项目引入了一种基于不同光学物理的新方法，有望实现更快、更精确的成像。该显微镜将用于研究变形虫的运动，它有望揭示原生质不同区域的受控硬化和流化，这些区域被认为是细胞运动的基础。

This project aims to improve the sensitivity and speed of Brillouin microscopes. Brillouin microscopes use light to measure the stiffness of samples in 3D without requiring physical access, allowing their use in inaccessible locations such as the interior of cells or within intact tissue. However, Brillouin microscopes are too slow to be used in most research. This project introduces a new approach based on different optical physics that is expected to enable faster and more precise imaging. The microscope will be used to study the movement of amoeba, where it is expected to reveal the controlled stiffening and fluidising of the different regions of protoplasm believed to underlie the cell mobility.