CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODIES

组织培养与单抗(TCMA)实验室的目标 保持这一核心设施的原始提案中概述的内容： 1.维持具成本效益的集中式细胞培养设施 与单抗/杂交瘤相关的活性可通过 对于劳伦斯和医疗中心的调查人员来说 MRDDRG。 2.协助研究人员研究原代细胞的生长和维持 用于各种分子和细胞的培养和细胞系 学习。 3.进行单抗的制备和鉴定 为MRDDRC调查人员提供支持，并为 研究项目需要持续供应的调查人员 抗体。 4.提供荧光激活的流式细胞术能力 特定细胞表型的分析和不同种类的 实验性治疗。

The goals for the Tissue Culture and Monoclonal Antibody (TCMA) Laboratory remain as outlined in the original proposal for this Core facility: 1. To maintain a cost-effective centralized facility where cell culture and monoclonal antibody/hybridoma-related activities can be performed by and for investigators at the lawrence and the Medical Center settings of the MRDDRG. 2. To assist investigators in the growth and maintenance of primary cell cultures and cell lines used in a variety of molecular and cellular studies. 3. To carry out generation and characterization of monoclonal antibodies for MRDDRC investigators and maintain hybridoma cell lines for the investigators whose research programs require a consistent supply of antibodies. 4. To provide fluorescence activated flow cytometry capabilities for analysis of specific cell phenotypes and the effects of various experimental treatments.