KN1 AND MAIZE FLORAL DEVELOPMENT

我这项研究的长期目标是了解 玉米的花发育。一个特定的目标是阐明这一功能 Kn1同源框基因在花发育中的作用。为此，我将 分析功能缺失的Kn1等位基因之间的双突变组合 和现有的花卉突变。KN1与克隆花的相互作用 基因将通过对这些基因产物的原位定位来确定 Kn1隐性突变植株。与Kn1相互作用的基因也将是 通过筛选隐性Kn1-1的抑制/增强子进行鉴定 R1等位基因。了解Kn1的调节和功能可能会提供 关于同源异型盒基因在其他系统中功能的线索。定义 KN1的同源结构域是KN1的同源结构域，KN1的下游靶点可能被证明是有价值的 与人类PRL基因相似，该基因已被认为是导致 前B细胞白血病。作为我长期目标的一部分，额外的基因 是花发育所需的，将通过筛选获得 携带突变转座子的玉米群体中的花突变。 这些基因的预测功能将通过分析 现有玉米的单突变表型和双突变表型 花的突变。因为新的突变是在转座子中获得的 有很大的可能性克隆该基因，从而 野生型基因产物的检测。

The long-term objective of my research is to understand the regulation of floral development in maize. A specific goal is to elucidate the function of the kn1 homeobox gene in floral development. To this end, I will analyze double mutant combinations between loss of function kn1-alleles and existing floral mutations. Interactions between kn1 and cloned floral genes will be determined by in situ localization of these gene products in kn1 recessive mutant plants. Genes that interact with kn1 will also be identified by screening for suppressers/enhancers of a recessive kn1- R1allele. Understanding the regulation and function of kn1 may provide clues as to the function of homeobox gene in other systems. Defining the downstream targets of kn1 may prove valuable as the homeodomain of KN1 is similar to that of the human PRL gene which has been implicated in causing pre-B cell leukemia. As part of my long-term goal, additional genes that are required for floral development will be obtained by screening for floral mutations among maize populations carrying the Mutator transposon. The predicted functions of these genes will be determined by analyzing the single mutant phenotypes and double mutant phenotypes with existing maize floral mutations. Because the new mutations are obtained in transposon lines, there is a strong probability of cloning the gene and thereby determining the wild type gene product.