INTERACTION OF PTO AND PTIL KINASES IN PLANT DISEASE RES

病原体和植物之间的相互作用代表了最重要的 植物生物学中的基本问题。克隆技术的最新进展 植物抗病基因的研究为我们的 植物携带抗性的分子机制研究 基因识别并保护自己免受病原微生物的侵害。这个 番茄丝氨酸/苏氨酸蛋白激酶PTO对赤霉病的抗性 由紫丁香假单胞菌引起的细菌性斑点病。番茄 表达avrPto。使用酵母双杂交系统，另一个 丝氨酸/苏氨酸蛋白激酶，命名为Pti1，与 已经确定了PTO。PTO体外特异性磷酸化Pti1 提示Pti1可能是PTO的重要组成部分- 导致细菌抗药性反应的信号通路。 本提案意在进一步审查这些措施的意义 通过提出两个问题得出的发现：1.疾病是否需要Pti1 耐药性？2.PTO与Pti1在体内有相互作用吗？建议数 实验内容包括：1.植物抗病性分析。 表达Pti1正义或反义RNA。二、审查 体内PTO和Pti1的物理相互作用。三、PTO的检测 体内的蛋白酪氨酸激酶活性和PTO、Pti1的磷酸化。这个 拟议中的研究有望扩大我们对该信号的了解 在植物与病原菌相互作用过程中发生的转导事件。

The interactions between pathogens and plants represent one of the most fundamental problems in plant biology. The recent progress in cloning of plant disease resistance genes has opened new avenues for our studying of the molecular mechanisms by which plant carrying resistance genes recognize and defend themselves against pathogenic microbes. The tomato serine/threonine protein kinase Pto confers resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato expressing avrPto. Using the yeast two-hybrid system, another serine/threonine protein kinase, designated Pti1, that interacts with Pto has been identified. Pto specifically phosphorylates Pti1 in vitro suggesting that Pti1 is likely to be an important component in the Pto- signaling pathway leading to the resistance response the bacterium. This proposal intends to further examine the significance of these findings by asking two questions: 1. Is Pti1 required for disease resistance? 2. Does Pto interact with Pti1 in vivo? The proposed experiments include: 1. Analysis of disease resistance in plants over- expressing the Pti1-sense or antisense RNA. II. Examination of the physical interaction of Pto and Pti1 in vivo. III. Detection of Pto kinase activity and the phosphorylation of Pto and Pti1 in vivo. The proposed research is expected to expand our knowledge of the signal transduction events which occur during plant-pathogen interactions.