BEHAVIORAL AND NEURAL ANALYSIS OF FEEDING IN APLYSIA

预设提案中描述的实验旨在 使用一个简单的 系统，软体动物加利福尼亚州的Aaplasia。既有吸收前（例如，救济 肠拉伸的）和吸收后（例如，血糖）因素将是 作为启动刺激进行研究。一旦启动刺激被 将采用所理解的电生理学技术 研究神经元的作用位点的信号相关的 启动刺激，使用半完整和体内制剂。在 此外，神经束追踪和神经染色的解剖技术 将被用来确定可能的传播途径，这些 刺激。一般原则源自于 了解这一过程中进食开始的生理学 一个简单的系统可以应用于脊椎动物的喂养系统 行为，无论是在健康还是患病状态下。

The experiments described in the preset proposal are designed to determine the mechanism for the initiation of feeding using a simple system, the mollusc Aplysia californica. Both preabsorptive (e.g., relief of gut stretch) and postabsorptive (e.g., blood glucose) factors will be investigated as initiating stimuli. Once the initiating stimuli are understood electrophysiological techniques will be employed to investigate the neuronal sites of action of the signals related to the initiating stimuli, using semi-intact and in vivo preparations. In addition, anatomical techniques of tract tracing and neural staining will be used to determine possible routes of transmission of these stimuli. It is the intention that general principles derived from understanding the physiology of the initiation of feeding in this simple system can be applied to vertebrate systems of feeding behavior, both in healthy and diseased states.