HYPOTHERMIA/ADENOSINE NEUROPROJECTION FOR BIRTH ASPHYXIA

缺氧缺血性脑病仍然是主要的神经系统疾病， 新生儿的并发症这次调查的长远目的 是发现新的治疗方法来防止出生造成的脑损伤 窒息及其毁灭性的后遗症本研究的具体目的 （1）评估缺血后或缺血后的神经保护作用。 轻度低温或腺苷增强新生大鼠幼崽（2）。 评估缺血后轻度脑缺血的联合神经保护作用， 低温和腺苷增强的新生大鼠幼崽。是 假设低温和Ado试剂单独地将 神经保护和组合将有更大的好处，因为 通过比较大脑半球重量，梗塞面积， 神经元形态评分和谷氨酸水平的改变，使用 光镜、免疫组化和神经化学微透析 适当地动物模型为21窝（10-12只幼仔/窝） 7日龄Sprague-Dawley大鼠幼仔，暴露于Rice Vannucci模型 缺氧缺血性损伤和各种恢复组合 条件与低温和腺苷（Ado）增效剂。这 研究希望阐明一个关键的临床问题， 热管理、腺苷增强和 神经保护

Hypoxic-ischemic encephalopathy remains the main neurological complication in the newborn. The long range purpose of this investigation is to discover new therapies to prevent brain damage due to birth asphyxia and its devastating sequelae. The specific aims of this study are: (1) To assess the neuroprotective effects of either postischemic mild hypothermia or adenosine potentiation in the neonatal rat pup (2). To assess the combined neuroprotective effects of postischemic mild hypothermia and adenosine potentiation in the neonatal rat pup. It is hypothesized that both hypothermia and Ado agents individually will be neuroprotective and in combination will have an even greater benefit, as measured by comparative hemispheric brain weights, infarct size, alterations in neuronal morphology scores and glutamate levels, using light microscopy, immunohistochemistry and neurochemistry microdialysis as appropriate. The animal model will be 21 litters (10-12 pups/litter) of 7 d old Sprague-Dawley rat pups, exposed to the Rice Vannucci model for hypoxic-ischemic insult and various combinations of recovery conditions with hypothermia and adenosine (Ado) potentiating agents. This research hopes to shed light on a crucial clinical question, regarding the relationship of thermal management, adenosine potentiation and neuroprotection.