LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES

这一建议旨在扩大高绩效的范围 液相色谱法及其在医学和生活中的应用 沿着沿着几条一致的路线发展科学。 的分子相互作用 参与固定相对生物聚合物的保留， 将研究表面介导的色谱过程 详细 本研究结果对疏水，静电 和生物特异性相互作用有望揭示 生物系统中的相关表面现象，并提供 用于表征所用各种固定相的方法 在实践中 将开展一项重大研究工作， 开发高效色谱分离技术 为了满足对聚糖及其结合物的迫切需求， 对于用于分析和纯化这些物质的合适工具， 其生物学意义正日益得到认可。 同时，将开发有效的方法， 待分离和纯化凝集素和寡糖 结合我们对色谱法的研究， 生物碳水化合物 这些程序的系统化 将允许建立一套单位业务， 从生物基质中分离复杂分子。 在这个项目的过程中获得的经验将用于广告 特别合作研究项目， 生命科学和医学的重要性。 的另一部分 拟议的研究将集中在蛋白质的快速分析， 核酸在填充有微膜吸附剂的柱上， 使用适当修改的仪器。 这种方法 承诺分析时间至少减少十倍。 基于大孔硅质和 聚合物微球将被开发用于分析 蛋白质、核酸和生物学上重要的糖; 在分析色谱方面取得的经验， 物质将用于制备吸附剂， 分居 非线性理论与实践研究 洗脱色谱法有望提供一个框架， 制备色谱分析与设计 过程

This proposal is aimed at expanding the scope of high performance liquid chromatography and its application to medicine and the life sciences along several congruos lines. The molecular interactions involved in the retention of biopolymers by stationary phases in surface mediated chromatographic processes will be investigated in detail. The results of this study on hydrophobic, electrostatic and biospecific interactions are expected to shed light on the relevant surface phenomena in biological systems and to provide a means for the characterization of various stationary phases used in practice. A major research effort will be undertaken to develop highly efficient chromatographic separation techniques for glycans and their conjugates in order to meet an acute need for suitable tools for analysis and purification of such substances, whose biological significance is being increasingly recognized. Concurrently, efficient methods will be developed for the isolation and purification of lectins and oligosaccharides to be used in conjunction with our research on chromatography of biological carbohydrates. Systematization of these procedures will permit the establishment of a set of unit operations for the isolation of complex molecules from biological matrices. Experience gained in the course of this project will be used in ad hoc collaborative research projects on separation problems of importance to the life sciences and medicine. Another part of the proposed research will focus on the rapid analysis of proteins and nucleic acid on columns packed with micropellicular sorbents and using appropriately modified instrumentation. This approach promises at least a ten-fold reduction in the time of analysis. Novel stationary phases based on macroporous siliceous and polymeric microspheres will be developed for the analysis of proteins, nucleic acids and biologically important sugars; the experience gained in the analytical chromatography of these substances will be used to prepare sorbents for their preparative separation. Studies on the theory and practice of non-linear elution chromatography are expected to provide a framework for the analysis and design of the preparative chromatographic process.