STRUCTURAL BIOLOGY OF THE GLYCINE RECEPTOR

描述：本提案的总体目标是 重组人α 1甘氨酸受体氯通道的测定。 甘氨酸受体是配体门控通道大家族的一部分， 介导突触处的信号传导。 一个成员的结构 这个家族，乙酰胆碱受体，已经在三个国家进行了研究。 尺寸和低分辨率结构， 然而，序列尚未映射到该结构中。 实验旨在区分合理的拓扑模型， 通过标记半胱氨酸残基， 甘氨酸受体与化学裂解试剂。 的一个主要目标 建议是结晶纯化的全息受体以及其 细胞外，配体结合域。 功能性甘氨酸受体组成 可以从重组蛋白重构，并且 是在co-P.I的实验室里生产的，产量足够大， 审判

DESCRIPTION: The overall goal of this proposal is the structure determination of recombinant human a1 glycine receptor chloride channel. The glycine receptor is part of a large family of ligand-gated channels that mediate signal transduction at the synapse. The structure of one member of this family, the acetylcholine receptor, has been studied in three dimensions by electron microscopy and a low resolution structure is available, however the sequence has not been mapped into this structure. Experiments are aimed at distinguishing plausible topological models for this family of channels by labeling cysteine residues engineered into the glycine receptor with chemical cleavage reagents. A major goal of the proposal is to crystallize purified holoreceptor as well as its extracellular, ligand binding domain. Functional glycine receptor composed of a single subunit type can be reconstituted from recombinant protein, and is produced in the co-P.I's lab in large enough yields for crystallization trials.