NMR OF HIV REVERSE TRANSCRIPTASE PRIMERS

描述：在本申请中，提出研究以确定 与反密码子相关的一系列分子的溶液结构 用于合成HIV负链的tRNA 3lys引物的环 DNA使用NMR。 PI将合成RNA 17聚体，对应于 反密码子环结构的茎环。 他将取代内 这一系列天然存在的修饰核苷在同一位点 它们出现在tRNA 3lys中。 这些变化对 茎环的结构、稳定性和RT结合将是 评估。 调查人员还表示，他打算进行调查 RNA修饰对RNA-RNA相互作用的影响， 对于引物与互补RNA模板的相互作用很重要。 传统的2D NMR方法沿着新的核磁共振技术， 异源3D NMR实验将用于指定RNA共振 并获取结构信息。 距离几何与分子 动力学计算方法将被用来转换NMR结构， 定义的3D结构。

DESCRIPTION: In this application, studies are proposed to determine the solution structures of a series of molecules related to the anticodon loop of the tRNA3lys primer for the synthesis of the minus strand of HIV DNA using NMR. The PI will synthesize RNA 17-mers corresponding to the stem-loop of the anticodon loop structure. He will substitute within this series naturally occurring modified nucleosides at the same site that they appear in tRNA3lys. The influence of these modifications on the structure, stability and RT binding of the stem loop will be evaluated. The investigator also states that he intends to investigate the effect of RNA modification on RNA-RNA interactions that may be important for primer interactions with complimentary RNA templates. Traditional 2D NMR methods along with newer homonuclear and heteronuclear 3D NMR experiments will be used to assign RNA resonances and to derive structural information. Distance geometry and molecular dynamics calculational methods will be used to convert NMR structural constraints into defined 3D structures.