IMAGING THE RESPONDING SI NEURONAL POPULATION

计划中的实验侧重于研究刺激诱发的模式。 躯体感觉皮层(SI)的活动，特别是 由重复的皮肤刺激引起的激活模式。在01年 年，使用C14-2DG代谢图方法的动物实验(2DG) 以及一种用于成像本征皮质的红外光学记录方法 将执行信号(红外成像)。目标是获得和 比较大脑皮层对同一刺激反应的IR和2DG图像 条件。在02-05年度，红外线光学记录技术将 用于表征对刺激引起的SI活性的影响 (I)NMDA受体阻断模式，(Ii)不同频率的 振动触觉刺激，以及(Iii)不同的时空模式 皮肤刺激。预计拟议的研究将 提供有关刺激措施后果的新颖而有用的信息- 空间分布的多个神经元之间的诱发相互作用 被皮肤刺激激活的人群。应提供的信息 应该会填补目前已知的关于 躯体感觉皮质对皮肤刺激的反应-可归因于差距 主要是由于方法学的不足，至少到目前为止， 已经通过实验得到了强调。此外，调查结果应该是 为正在进行的刺激建模和模拟研究提供指导- 定向躯体感觉皮层网络动力学及其基础 神经机制。

The proposed experiments focus on studies of patterns of stimulus evoked activity in somatosensory cortex (SI), and in particular, on the activation patterns evoked by repetitive skin stimulation. In the 01 year, animal experiments using the C14-2DG metabolic mapping method (2DG) and the infrared optical recording method for imaging intrinsic cortical signals (IR imaging) will be performed. The goal is to obtain and compare IR and 2DG images of the cortical response to the same stimulus conditions. In years 02-05, the IR optical recording technique will be employed to characterize the effects on stimulus evoked SI activity patterns of (i) NMDA receptor block, (ii) different frequencies of vibrotactile stimulation, and (iii) differing spatiotemporal patterns of skin stimulation. It is anticipated that the proposed studies will provide novel and useful information about the consequences of stimulus- evoked interactions among the multiple and spatially distributed neuronal populations activated by cutaneous stimuli. The information to be obtained should fill major gaps in what is currently known about the somatosensory cortical response to skin stimulation-gaps attributable principally to inadequacies of the methodologies which, at least to date, have been emphasized by experiments. In addition, the findings should provide direction to ongoing modeling and simulation studies of stimulus- directed somatosensory cortical network dynamics and the underlying neural mechanisms.