RNA CAPPING IN TRYPANOSOMES

描述（改编自申请人摘要）：拟议的研究 主要研究锥虫中的RNA加帽反应。 研究者已 从束状短翅虫中纯化了加帽酶（鸟苷酰转移酶 并从C.束状体和锥虫 布鲁塞。 拟议研究的长期目标是了解 的加帽反应的RNA特异性的机制和基础， 锥虫 研究者提出了三个具体目标：（1） 进行锥虫加帽酶的结构-功能分析; (2)评估加帽酶基因耗竭的后果; 和（3）寻找与细胞相互作用的细胞成分， 加帽酶

DESCRIPTION (Adapted from the Applicant's Abstract): The proposed research focuses on the RNA capping reaction in trypanosomes. The investigator has purified the capping enzyme (guanylyltransferase) from Crithidia fasciculata and has cloned the corresponding genes from C. fasciculata and Trypanosoma brucei. The long-term goal of the proposed research is to understand the mechanism and the basis for the RNA specificity of the capping reaction of trypanosomatids. The investigator proposes three specific aims: (1) to undertake a structure-function analysis of the trypanosome capping enzyme; (2) to assess the consequences of genetic depletion of the capping enzyme; and (3) to search for cellular components that physically interact with the capping enzyme.