MEASUREMENT OF ALBUMIN SYNTHETIC RATE DURING RECOVERY FROM INTENSE EXERCISE

我们测量了从剧烈的肝炎恢复期间肝脏白蛋白的合成。 先前显示的间歇性运动方案可扩大血浆容量。 使用一种稳定的同位素示踪剂持续注入 苯丙氨酸、肝脏合成分数(FSR)和绝对值 白蛋白的合成速率(ASR)由富集度测定 白蛋白和游离血管内前体库中的苯丙氨酸。 白蛋白FSR和ASR分别为6.39±0.48%和120±9 mg(KgBW)~(-1)。 分别在运动后3~6小时恢复；FSR和ASR在 时间控制研究日分别为5.94±0.47%和104±9 mg(KgBW)~(-1) 分别为第1天。FSR和ASR分别增加6%和16%(P&lt；0.05) 运动后血浆白蛋白含量升高 在恢复5小时和6小时(P&lt；0.05)时，总蛋白质含量增加 整个恢复(P&lt；0.05)和负的自由水清除(P&lt；0.05) 与基准值相比，恢复时间分别为2、3和6.5小时； 在时间控制研究中，变量与基线没有变化 天。白蛋白含量增加，游离水清除率降低 有助于在剧烈运动后将液体滞留在循环内 锻炼身体。测量到的白蛋白合成增加并不能解释 运动后恢复期间白蛋白含量的全部增加。 尽管测量到的白蛋白合成增加并不能解释 在运动后6小时内血浆白蛋白含量升高，我们 得出的结论是，它被抬高到足以解释增加的 恢复24小时后测量白蛋白含量(在先前的研究中)。

We measured hepatic albumin synthesis during the recovery from an intense intermittent exercise protocol previously shown to expand plasma volume. Using a primed-constant infusion of a stable isotopic tracer of phenylalanine, hepatic fractional synthetic rate (FSR) and absolute synthetic rate (ASR) of albumin were determined from the enrichment of phenylalanine in albumin and in the free intravascular precursor pool. Albumin FSR and ASR were 6.39+0.48% day-1and 120 +9mg (kgBW)-1 day-1, respectively, at 3 to 6 hours recovery from exercise; the FSR and ASR on the time control study day were 5.94+0.47% day-1 and 104+9mg (kgBW) -1 day-1 respectively. The 6% and 16% increases (P<0.05) in FSR and ASR following exercise were associated with an elevated plasma albumin content at 5 and 6 hours recovery (P<0.05), an increased total protein content throughout recovery (P<0.05), and a negative free water clearance (P<0.05) at 2,3 and 6.5 hours of recovery when compared to baseline values; these variables were unchanged from their baselines on the time control study day. Increased albumin content and reduced free water clearance both contribute to a retention of fluid within the circulation after intense exercise. The measured increase in albumin synthesis did not account for the entire increase in albumin content during recovery from exercise. Although the measured increase in albumin synthesis did not account for the elevated plasma albumin content in the 6 hours following exercise, we concluded that it was elevated sufficiently to account for the increased albumin content measured (in a prior study) after 24 hours of recovery.