COMPUTER ASSISTED STEREOLOGY FOR MOUSE BRAIN DATABASES

需要方法来提取准确，可靠的定量数据， 大脑结构迅速大幅增加，特别是随着 作为C.N.S.模型的遗传操作小鼠的增殖 紊乱在我们的人脑项目P20下开发的软件工具 协会MH/DA 52154定义了正常和 神经艾滋病和其他动物模型中的病理性脑功能 神经退行性模型。在第一阶段STTR申请中，我们将：1） 扩展我们现有的NeuroZoom计算机辅助显微镜应用 与其他软件体视学探头; 2）开发跨平台 访问定量神经解剖数据库; 3）启动 小鼠脑体视学数据库的建立 正常大脑中的结构;以及4）对正常大脑中的结构进行原型分析。 脊髓神经变性的转基因小鼠模型，以记录 这种体视学方法在遗传病理学中的可行性- 操纵老鼠 我们设想了三类潜在的商业客户， 产品：1）拥有足够专业知识的实验室， 分析内部与我们的软件，但需要不断访问我们的 与我们自己的突变小鼠进行比较的规范数据库; 2） 开发转基因小鼠品系的生物技术公司或实验室 和外包的全面结构特点;以及 3)制药公司，需要协助开发现场 他们的分析程序，并要求访问规范数据库。 拟定商业应用： 没有可用的软件，包括映射，体视学， 数据库、互联网接入、3D可视化和神经电路数据， 一个包裹。这两种选择也没有体视学数据库 小鼠大脑的区域，或用于转基因小鼠。有三个班 潜在客户：l）拥有足够专业知识的实验室， 使用我们的软件进行内部分析; 2）生物技术 实验室开发转基因小鼠和外包， 全面的结构表征;和3）制药公司 他们需要帮助来开发现场分析程序。

The need for methods to extract accurate, reliable quantitative data on brain structure rapidly has increased substantially, especially with the proliferation of genetically manipulated mice as models_of C.N.S. disorders. Software tools developed under our Human Brain Project P20 consortium MH/DA 52154 define the structural bases for normal and pathologic brain function in animal models of Neuro-AIDS and others neurodegenerative models. In this Phase I STTR application, we will: 1) extend our existing NeuroZoom computer-assisted microscopy application with additional software stereological probes; 2) develop cross-platform access to a quantitative, neuroanatomic database; 3) initiate the development of a stereologically-derived database of key mouse brain structures in the normal brain; and 4) perform a prototypic analysis of a transgenic mouse model of spinal neurodegeneration to document the feasibility of such stereological approaches to pathology in genetically- manipulated mice. We envision three classes of potential commercial customers for these products: 1) laboratories possessing sufficient expertise to carry out the analyses in-house with our software, but require ongoing access to our normative database against which to compare our own mutant mice; 2) biotechnology firms or laboratories developing lines of transgenic mice and outsourcing for their comprehensive structural characterization; and 3) pharmaceutical companies that need assistance in developing an on-site program for their analyses, and require access to the normative database. PROPOSED COMMERCIAL APPLICATION: There is no available software that incorporates mapping, stereology, databases, Internet access, 3D visualization, and neurocircuitry data in one package. There is also no stereology database for either select regions of the mouse brain, or for a transgenic mouse. There are 3 classes of potential customers: l) laboratories possessing sufficient expertise to carry out the analyses in-house with our software; 2) biotechnology laboratories developing transgenic mice and outsourcing for their comprehensive structural characterization; and 3) pharmaceutical companies that need assistance in developing an on-site program for their analyses.