INTERPHASE CHROMOSOME CONDENSATION FOR CYTOGENETICS

这个项目的长期目标是开发出能够 能够分析间期的结构染色体异常 细胞。眼下的目标是通过以下方式检测和列举易位 自动图像分析可检测极低频率的易位 在正常细胞的背景下。第一阶段的具体目标是 1)开发一种使用有丝分裂产物的体外技术 细胞和/或蛙卵母细胞浓缩不固定间期的染色质 核移入整条染色体，用于染色体畸变的计数 全染色体涂染探针荧光原位杂交， 2)产生高密度的这种浓缩的染色体和/或核 在显微镜载玻片上进行自动图像分析，3)修改 用于自动成像的现有网点计数软件 间期细胞浓缩染色体分析，4)定量 通过自动图像分析间期细胞中的易位。 从这个项目开发的方法应该适用于 肿瘤活检中染色体异常的检测和细针 以及潜在诱变物/致癌物的筛选和 辐射防护剂。预计含有这些试剂的试剂盒 染色体浓缩最终可以作为研究和营销 临床产品。 拟议的商业应用：环境诱变；早期癌症 诊断和预后。

The long-term objective of this project is to develop methods that will enable the analysis of structural chromosome aberrations in interphase cells. The immediate goal is to detect and enumerate translocations by automated image analysis to detect very low frequencies of translocations against a background of normal cells. The Specific Aims of Phase I are 1) to develop an in vitro technique employing the products of mitotic cells and/or frog oocytes to condense the chromatin of unfixed interphase nuclei into whole chromosomes for enumeration of aberrations by fluorescence in situ hybridization with whole chromosome painting probes, 2) to produce high densities of such condensed chromosomes and/or nuclei on microscope slides to permit automated image analysis, 3) to modify existing dot.counting software for application to the automated image analysis of condensed chromosomes in interphase cells, 4) to quantify translocations in interphase cells by automated image analysis. The methodology developed from this project should be applicable to the detection of chromosome aberrations in tumor biopsies, and fine needle aspirates as well as the screening of potential mutagens/ carcinogens and radioprotectants. It is anticipated that kits containing these reagents for chromosome condensation could be ultimately marketed as research and clinical products. PROPOSED COMMERCIAL APPLICATION: Environmental mutagenesis; cancer early diagnosis and prognosis.