DEVELOPMENT OF AN IMMUNOLOGICALLY BASED COCKROACH ANTIGEN DETECTION SYSTEM

美国农业部和国防部的调查人员继续 分享对蟑螂潜在免疫活性的兴趣 经过各种杀虫剂和杀虫剂处理后留在住宅中的碎片 清洁剂。蟑螂残留物的冻干提取液和 兔抗血清池，由LST制备，提供来源材料 由ARS，现在是一个酶联免疫吸附试验系统的参考。今年, 用酶联免疫吸附试验检测暴露时间的样品；结果直接 与接触时间长短有关。ARS的两个模拟厨房是 通过拭子测量面积进行调查；提取拭子，进行检测 并与定时暴露样本进行了酶联免疫吸附试验。厨房区域 已经用几种方法和拭子/ELISA法进行了清洗 已经重复了一次，以验证抗原已经被移除。平面图 已经被征召将已知数量的蟑螂释放到 厨房区域，并重复研究。我们已经被接洽，希望分享 该系统与巴尔的摩约翰·霍普金斯大学的过敏症/哮喘研究人员合作。

Investigators at the USDA and the Department of Defense have continued to share interest in the potential immunological activity of cockroach debris left in dwellings after treatment with various insecticides and cleaning agents. The freeze-dried extracts of Cockroach debris and a pool of rabbit antiserum, prepared by LST with source material provided by ARS, are now the references of an ELISA system. This year, exposure-timed samples were assayed by ELISA; results were directly correlated to length of exposure. Two mock kitchens at ARS were surveyed by swabbing measured areas; the swabs were extracted, assayed and related to the timed exposure samples by ELISA. The kitchen areas have been cleaned using several methods, and the swabbing/ELISA process has been repeated to verify that the antigen has been removed. Plans have been drafted to release a known quantity of Cockroaches into the kitchen areas and repeat the studies. We have been approached to share the system with Allergy/Asthma researchers at Johns Hopkins in Baltimore.