MOLECULAR BASIS OF TRANSCRIPTIONAL ACTIVATION BY TFIIIA

产品说明： 在这个提议中，达比博士描述了 重点是参与征聘的机制 转录机器的5S rRNA基因的特定因素 TFIIIA。 已经鉴定了一种蛋白质，TIP 21，其与 TFIIIA的C末端。 该蛋白质与人源性前列腺素具有同源性， 酵母RNA聚合酶II和III的亚基。 达比医生希望 测试TIP 21在5SrRNA基因转录中任何功能作用 体外和体内使用标准方法（免疫耗竭， 核酶切除）并检查结构-功能（Far-Western， 两个混合物）。

DESCRIPTION: In this proposal, Dr. Darby describes experiments focused on the mechanisms involved in the recruitment of the transcriptional machinery to a 5S rRNA gene by the specific factor TFIIIA. A protein has been identified, TIP21 which interacts with the C-terminus of TFIIIA. This protein has homology with individual subunits of both yeast RNA polymerase II and III. Dr. Darby wishes to test any functional role of TIP21 in 5S rRNA gene transcription in vitro and in vivo using standard approaches (immunodepletion, ribozyme ablation) and to examine structure-function (Far-Western, two hybrid).