GRAVITROPIC SIGNAL TRANSDUCTION IN ARABIDOPSIS

重力是主要环境信号之一，决定了 许多植物的根和地上部的生长方向。这个 引力反应包括对重力信号的感知， 信号的传输，以及响应程序的执行，该响应程序重新 引导增长。因此，向重力性构成了植物的信号转导 路径。众所周知，这一途径依赖于对 植物激素生长素。拟议的研究是我们努力的延续 识别和鉴定与重力信号有关的基因 拟南芥中的转导。在这里，我们将重点介绍进一步的 T-DNA标记突变体的分析。我们将继续进行生理学和 我们先前分离的突变体的分子遗传学特征 确定受突变影响的基因的功能作用。 野生型等位基因的分子克隆和鉴定将进一步 定义这些基因的作用。野生型克隆将重新引入 农杆菌介导法稳定转化拟南芥的研究 用于突变表型的互补。遗传和分子 这些方法将被用来阐明这些基因与每个基因之间的相互作用 其他和之前发现的与向重力性有关的基因。 将利用稳态和暂态相变实验 证明改变正常表达的生物学效应 这些基因的模式。这项工作将为我们提供对 向重力性和生长素介导信号的分子机制 植物中的转导。

Gravity is one of the primary environmental signals determining the orientation of root and shoot growth in many plant species. The gravitropic response involves perception of the gravity signal, transmission of the signal, and execution of a response program that re- orients growth. Thus gravitropism constitutes a plant signal transduction pathway. This pathway is known to depend on transport and reception of the plant hormone auxin. The proposed research is a continuation of our effort to identify and characterize genes involved in gravitropic signal transduction in Arabidopsis thaliana. Here we will focus on further analysis of T-DNA-tagged mutants. We will continue the physiological and molecular genetic characterization of our previously isolated mutants to establish the functional roles of the genes affected by the mutations. Molecular cloning and characterization of wild-type alleles will further define the roles of these genes. Wild-type clones will be re-introduced into Arabidopsis by Agrobacterium-mediated stable transformation to test for complementation of mutant phenotypes. Genetic and molecular approaches will be used to elucidate interactions of these genes with each other and with previously identified genes involved in gravitropism. Stable and transient transformation experiments will be used to demonstrate the biological effects of altering the normal expression patterns of these genes. This work will provide new insights into the molecular mechanisms of gravitropism and of auxin-mediated signal transduction in plants.