INTRACELLULAR RNA SORTING, TRANSPORT AND LOCALIZATION

RNA分选、靶向mRNA转运和局部蛋白的重要性 由于信息的汇集， 从研究集中在各种分子，细胞和 发展问题。在过去的15年里， 细胞内定位RNA已经在多种细胞中被鉴定， 在脊椎动物和无脊椎动物中，从卵母细胞到 上皮细胞到神经元和神经胶质。此外，不同类别的 蛋白质由定位的RNA编码，包括转录因子， RNA结合蛋白，细胞骨架蛋白，细胞内信号传导 分子和分泌的生长因子。现在很明显， 特定的细胞内定位在 包括卵母细胞极性建立的生物学过程， 早期胚胎分化，体细胞极化和功能， 突触生长和脊椎动物神经系统的可塑性。 拟议会议的目标是建立在两个非常成功的 1994年和1996年举行的关于该专题的FASEB夏季会议， 汇集了具有广泛背景的研究人员， 研究RNA分选和定位的观点， 实验方法。目的是探讨共同的主题， 问题包括：（i）RNA定位在建立 无脊椎动物的胚胎细胞谱系和轴向极性， 脊椎动物;（ii）顺式肌动蛋白信号和反式作用的性质 将mRNA靶向特定细胞内定位的因子;（iii） 所涉运载系统的性质;（四）机制 一旦它们到达其适当的目的地，锚定位的mRNA;（v） 不同目的地的性质和数量的定义 在单个细胞类型内;（vi）mRNA易位的程度 并且分选受到细胞外和细胞内信号的调节 （vii）参与RNA从细胞中转运的机制。 在某些情况下从细胞质到细胞核; 和（viii）RNA分选在细胞内起作用的多种方式 功能

The importance of RNA sorting, targeted mRNA transport, and local protein synthesis has become evident as a result of the convergence of information from studies focusing on a variety of molecular, cellular and developmental questions. During the past fifteen years, over seventy intracellularly localized RNAs have been identified in a diversity of cell types in both vertebrates and invertebrates, ranging from oocytes to epithelial cells to neurons and glia. In addition, diverse classes of proteins are encoded by localized RNAs, including transcription factors, RNA-binding proteins, cytoskeletal proteins, intracellular signaling molecules and secreted growth factors. It is now clear that the targeting of mRNAs to particular intracellular localizations play a key role in biological processes that include the establishment of oocyte polarity, early embryonic differentiation, somatic cell polarization and function, synapse growth, and plasticity in the vertebrate nervous system. The goal of the proposed conference is to build on two highly successful FASEB summer conferences on the topic that were held in 1994 and 1996, by bringing together researchers with a wide range of backgrounds and perspectives who study RNA sorting and localization using a variety of experimental approaches. It is the intent to explore common themes and questions including: (i) The role of RNA localization in the establishment of embryonic cell lineages and axial polarity in invertebrates and vertebrates; (ii) the nature of the cis-actin signals and trans-acting factors that target mRNAs to particular intracellular localizations; (iii) the nature of the delivery systems that are involved; (iv) the mechanisms that anchor localized mRNAs once they reach their proper destinations; (v) definition of nature and number of different destinations that reside within individual cell types; (vi) the extent to which mRNA translocation and sorting is regulated by extracellular and intracellular signaling processes; (vii) the mechanisms involved in transport of RNAs from the nucleus to the cytoplasm in some cases from the cytoplasm to the nucleus; and (viii) the multiple ways in which RNA sorting plays a role in cellular function.