NITRIC OXIDE EFFECT ON HIV 1 REPLICATION

描述：(改编自《调查者摘要》)本申请旨在 研究细胞因子诱导的NO是否会影响HIV-1前病毒 综合。为了实现这一点，具体目标是构建 Tat调控下具有诱导型一氧化氮合酶的逆转录病毒载体。 在目标2中，应用程序将分析一氧化氮合成酶的影响 关于艾滋病毒复制的过度表达。在目标3中，应用程序将检查 一氧化氮对HIV-1前病毒整合及HIV转录的影响 长期感染的线路。目标4是确定NO对 人类免疫缺陷病毒1型干扰素耐药株的表达。这些研究可能会导致 艾滋病毒复制的新疗法。

DESCRIPTION: (Adapted from Investigator's Abstract) This application aims to study whether NO induced by cytokines can affect HIV-1 provirus synthesis. To accomplish this, the specific aims are to construct retroviral vectors which have inducible NO synthetase under tat regulation. In Aim 2, the application will analyze the effect of NO synthetase over-expression on HIV replication. In Aim 3, the application will examine the effect of NO on integrated HIV-1 provirus and HIV transcription in chronically-infected lines. Aim 4 is to determine the NO effect on the expression of IFN-a resistant HIV-1 isolates. These studies might lead to new therapies for HIV replication.