GRAVITROPIC SIGNAL TRANSDUCTION IN ARABIDOPSIS

重力是决定环境质量的主要环境信号之一。 许多植物的根和枝的生长方向。的 向重力反应涉及对重力信号的感知， 信号的传输，以及响应程序的执行， 定向增长。因此，向重力性构成了植物的信号转导 通路已知这一途径依赖于细胞的运输和接收， 植物激素生长素拟议中的研究是我们努力的继续 鉴定和表征与向重力信号有关的基因， 在拟南芥中的转导。在这里，我们将进一步关注 T-DNA标记的突变体的分析。我们将继续进行生理和 我们以前分离的突变体的分子遗传特征， 确定受突变影响的基因的功能作用。 野生型等位基因的分子克隆和表征将进一步 这些基因的作用。野生型克隆将被重新引入 通过农杆菌介导法转化拟南芥进行稳定转化试验 用于突变表型的互补。 遗传和分子 方法将用于阐明这些基因与每个 其他基因和先前鉴定的与向地性有关的基因。 稳定和瞬态转化实验将用于 证明了改变正常表达的生物学效应 这些基因的模式。这项工作将提供新的见解， 向重力性和生长素介导信号的分子机制 植物中的转导。

Gravity is one of the primary environmental signals determining the orientation of root and shoot growth in many plant species. The gravitropic response involves perception of the gravity signal, transmission of the signal, and execution of a response program that re- orients growth. Thus gravitropism constitutes a plant signal transduction pathway. This pathway is known to depend on transport and reception of the plant hormone auxin. The proposed research is a continuation of our effort to identify and characterize genes involved in gravitropic signal transduction in Arabidopsis thaliana. Here we will focus on further analysis of T-DNA-tagged mutants. We will continue the physiological and molecular genetic characterization of our previously isolated mutants to establish the functional roles of the genes affected by the mutations. Molecular cloning and characterization of wild-type alleles will further define the roles of these genes. Wild-type clones will be re-introduced into Arabidopsis by Agrobacterium-mediated stable transformation to test for complementation of mutant phenotypes. Genetic and molecular approaches will be used to elucidate interactions of these genes with each other and with previously identified genes involved in gravitropism. Stable and transient transformation experiments will be used to demonstrate the biological effects of altering the normal expression patterns of these genes. This work will provide new insights into the molecular mechanisms of gravitropism and of auxin-mediated signal transduction in plants.