DEVELOPMENT OF AN IN VITRO ESTROGEN MODULATOR ASSAY

描述(改编自摘要)由以下原因引起的健康后果 预计会接触到外来雌激素和抗雌激素。因为 潜在的对人类健康的影响，监管机构现在正在考虑 对具有潜在环境和潜力的工业化学品的要求 对人体暴露的雌激素活性进行评估。美国国家安全局 1997年6月25日通过了《饮水法》，其中包括筛选 饮用水中含有模拟雌激素的化学物质。有一个广告 需要合同服务来测试工业和环境化学品 对雌激素的调节作用。这项建议的目的是 建立一种独特的、新颖的和相关的体外检测方法，可用于 工业化学品和环境样品的筛选 雌激素调节活性。这种化验方法使用了一种对雌激素有反应的 人乳腺癌细胞系MCF-7细胞。初步研究表明 雌激素暴露会导致多细胞结节或 MCF-7细胞融合培养中的病灶。这些焦点的发展 似乎是雌激素特异性的，并被抗雌激素抑制。这个 第一阶段研究计划的具体目标是开发 雌激素依赖性病灶形成的优化条件。一种标准 将制定协议，通过以下方式优化条件 对所有相关技术因素进行系统评估。这些遗嘱 包括选择组织培养用品和条件、血清 要求和浓度、化验格式和评估、时间和 细胞增殖参数、高亲本的产生和选育 敏感稳定的MCF-7克隆。 建议的商业应用： 潜在的商业应用程序不可用。

DESCRIPTION (adapted from abstract) Health consequences resulting from exposure to xenoestrogens and antiestrogens are expected. Because of the potential human health effects, regulatory agencies are now considering the requirement for industrial chemical with potential environmental and human exposure to be evaluated for their estrogenicity. The US Safe Drinking Water Act was passed on June 25, 1997 which included screening of drinking waters for chemicals that mimic estrogen. There is a commercial need for contract service to test industrial and environmental chemicals for estrogen modulating potential. The objective of this proposal is to develop a unique, novel and relevant in vitro assay which can be used for the screening of industrial chemicals and environmental samples for estrogen modulation activity. The assay employs an estrogen responsive human breast cancer cell line, MCF-7 cells. Preliminary studies have shown that estrogen exposure results in development of multicellular nodules or foci in confluent cultures of MCF-7 cells. The development of these foci appears to be estrogen specific and is suppressed by antiestrogens. The specific aim of the Phase I research program is the development of optimized conditions for estrogen dependent focus development. A standard protocol will be developed in which conditions are optimized through systematic evaluation of all relevant technical factors. These will include selection of tissue culture supplies and conditions, serum requirement and concentration, assay format and evaluation, timing and cell proliferation parameters, and generation and selection of highly sensitive and stable MCF-7 clones. PROPOSED COMMERCIAL APPLICATIONS: Potential commercial application not available.