BLOOD PRESSURE CONTROL BY FOREBRAIN & BRAINSTEM NEURONS

描述：（改编自申请人的摘要）长期目标 这项研究的目的是描述控制中枢神经系统的通路， 基底交感神经放电（SND）和心血管功能。 自1990年以来，PI研究了10 Hz节奏的控制， 是去大脑或氨基甲酸乙酯中SND的主要特征- 麻醉猫这种节奏在神经中是高度连贯的 控制多种心血管靶器官，可能是一种 诱发脊髓交感神经复合模式的先决条件 伴随着一些行为状态的流出。研究期间， 目前的资助期是第一次确定具体的中枢神经系统 规范这种活动的网站。逆向激活数据 实验表明，一些相互之间的联系， 这些区域和药理学研究表明神经递质 在SND中控制10 Hz节律的重要系统。 在拟议的5年研究计划中， 分析技术将与微离子导入法相结合 和微量注射药物，以解决4个具体目标：（1）测试 假设A5、A6和臂旁/Kolliker- 10 Hz节律的控制主要涉及神经元复合体（2） 识别相互联系和迹象（兴奋或抑制） 脑干神经元之间的突触连接 与SND的10 Hz节律相关，（3）为了检验假设， 10 Hz节律是由脑干的分布式网络产生的， 神经元，和（4）以确定中央网站，其中多巴胺能和 儿茶酚胺能神经元的作用是影响10 Hz 节律和不规则的2-6 Hz振荡。这些研究 将加深我们对负责 用于在静息SND中产生和控制10 Hz节律。

DESCRIPTION: (adapted from applicant's abstract) The long-range goal of this research is to characterize the CNS pathways that control basal sympathetic nerve discharge (SND) and cardiovascular function. Since 1990, the PI has studied the control of the 10-Hz rhythm that is the predominant feature of SND in decerebrate or urethane- anesthetized cats. This rhythm is highly coherent in nerves controlling a variety of cardiovascular target organs and may be a prerequisite for inducing the complex patterns of spinal sympathetic outflow that accompany some behavioral states. Studies during the current funding period are the first to identify specific CNS sites that regulate this activity. Data from antidromic activation experiments have indicated reciprocal connections between some of these areas and pharmacologic studies have suggested neurotransmitter systems that are important in the control of the 10 hz rhythm in SND. In the proposed 5-yr research plan, state-of-the-art electrophysiologic and analytic techniques will be combined with microiontophoresis and microinjection of drugs to address 4 Specific aims: (1) To test the hypothesis that pontine neurons in the A5, A6 and parabrachial/Kolliker- Fuse complex are involved in the control of the 10 Hz rhythm, (2) To identify the interconnections and signs (excitation or inhibition) of synaptic connections among brainstem neurons with activity correlated to the 10 Hz rhythm in SND, (3) To test the hypothesis that the 10 Hz rhythm is generated by a distributed network of brainstem neurons, and (4) to identify the central sites where serotonergic and catecholaminergic neurons act to affect the balance between the 10 Hz rhythm and the irregular 2-6 Hz oscillations in SND. These studies will enhance our understanding of the central circuitry responsible for generating and controlling the 10 Hz rhythm in resting SND.