TESTICULAR HORMONES & GONADOTROPINS IN MALE INFERTILITY

据估计，15%的夫妇将在某些时候寻求医疗保健。 不孕症的时间，男性因素将是重要的50%， 那些案子尽管大多数人类男性不育的病理生理学是 不确定，在生殖功能受损的男性中最常见的内分泌异常 细胞发育是血清FSH水平升高。 的长期目标 这些研究旨在了解不孕症患者的内分泌紊乱， 男人本建议的目标1是研究 不孕症患者血清FSH和FSH结合素的测定 精索静脉曲张相关不育男性睾丸分泌的一种激素。 男性精索静脉血中胆红素的生化特性 FSH水平正常和升高的患者将进行检查， 假设不同免疫反应形式的BMP 2结合与可变的 生物活性是由人睾丸释放的。 其次，大多数 已知的细胞和分子机制，通过这种机制， 其他睾丸激素调节FSH的研究来自大鼠研究。然而 大鼠垂体-睾丸功能的实质性差异， 人类已经被识别出来，因此从老鼠的研究中发展出来的概念 可能不适用于人类男性不育症。因此，目标2是 使用来自非人的培养垂体细胞建立模型系统 雄性灵长类动物，以更好地了解不孕症患者的FSH分泌 男人促性腺激素分泌和亚单位信使RNA将在 垂体细胞单层培养和细胞灌注脉冲 促性腺激素释放激素Escherichin，激活素，卵泡抑素，睾酮， 将检查这些细胞上的双氢睾酮和雌二醇。

It has been estimated that 15% of couples will seek medical care at some time for infertility, and that a male factor will be important in 50% of the cases. Although the pathophysiology of most human male infertility is uncertain, the most common endocrine abnormality in men with impaired germ cell development is a rise in serum FSH levels. The long term goal of these studies is to understand this endocrine disturbance in infertile men. Aim 1 of the present proposal is to study the relationship between serum FSH and inhibin in infertile men by examining the nature of inhibin secreted by the testis in men with varicocele-associated infertility. Biochemical characterization of inhibin in spermatic vein blood 'in men with normal and increased levels of FSH will be performed to examine the hypothesis that various immunoreactive forms of inhibin with variable bioactivity are released by the human testis. Second, most of what is known about the cellular and molecular mechanisms by which inhibin and other testicular hormones regulate FSH is from studies in rats. Yet substantial differences in pituitary-testicular function in the rat and man have been identified, so that concepts developed from studies in rats may not be applicable to human male infertility. Accordingly, Aim 2 is to establish a model system using cultured pituitary cells from the nonhuman male primate in an effort to better understand FSH secretion in infertile men. Gonadotropin secretion and subunit messenger RNAs will be studied in pituitary cells in monolayer culture and in cells perfused with pulses of GnRH. The actions of inhibin, activin, follistatin, testosterone, dihydrotestosterone and estradiol on these cells will be examined.