T CELL ACTIVATION IN HIV 1 REVERSE TRANSCRIPTION/INTEGRA

描述：（改编自研究者摘要）在本提案中， 一位年轻的研究者希望研究是否有蛋白质参与T细胞活化 可能用于HIV-1逆转录。 便利她 研究中，她获得的初步数据表明，T细胞 蛋白质结合生物素化的HIV-1单链DNA和RNA构建体 使用电泳迁移率偏移的非放射性适应 比色法 使用这种技术，研究人员建议调查 HIV-1逆转录中间产物与T细胞相互作用 蛋白质来检验早期和有效逆转 HIV-1 ssDNA中间体的转录可能有助于HIV-1的感染 通过为参与的宿主细胞蛋白提供结合位点的方法， 整合前或整合事件。 为了实现这一目标，研究人员 具体目标：具体目标1： HIV-1 RNA、单链DNA、RNA DNA杂交构建体上的特异性位点 与T细胞蛋白相互作用。 具体目标2：调查 HIV-1逆转录中间体与HIV-1之间的相互作用 整合酶蛋白

DESCRIPTION: (Adapted from investigator's abstract) In this proposal, a young investigator wishes to study if proteins involved in T cell activation might be used in the HIV-1 reverse transcription. To facilitate her studies, she has obtained preliminary data demonstrating that T cell proteins bind to biotinylated HIV-1 single stranded DNA and RNA constructs using a non-radioactive adaptation of the electrophoretic mobility shift assay. Using this technique, the investigator proposes to investigate the interaction of HIV-1 reverse transcription intermediates with T cell proteins to test the hypothesis that early and efficient reverse transcription of HIV-1 ssDNA intermediates may assist HIV-1 in the infection process by providing a binding site for host cell proteins involved in pre-integration or integration events. Towards this goal, the investigator proposes the following Specific Aims: Specific Aim 1: To delineate the specific sites on HIV-1 RNA, single stranded DNA, RNA DNA hybrid constructs interacting with T cell proteins. Specific Aim 2: To investigate interactions between HIV-1 reverse transcription intermediates and the HIV-1 integrase protein