WOUND HEALING MODEL USING ENDOTHELIAL CELL CULTURES

在成功的伤口愈合事件中，内皮细胞邻近 到机械性伤口的地方必须察觉到伤害 发生；迁移到该地区；扩散以恢复 有缺陷的区域；最后，回到非增殖状态。 这一过程涉及到许多基因的调控。为了澄清 这些基因，一种使用内皮细胞的体外伤口愈合模型 细胞培养，维持在人工基底膜上 IV型胶原蛋白正在开发中。差异显示将 用于分离和识别受调控的mRNAs 在这个伤口愈合的过程中。这种方法，以及 使用感染性心内膜炎的体内模型可以提供 对治愈的潜在疾病机制的洞察 心脏瓣膜受伤或患病。它可能会成为 血小板增殖体的发育。差异基因 这种相对无血管的伤口愈合过程中的调节， 胶原性瓣膜组织，可能在决定 修复或形成植被。 关键词：伤口愈合，内皮细胞，分化 显示为感染性心内膜炎。

In a successful wound healing event, endothelial cells adjacent to a site of mechanical wound must perceive that an injury has occurred; migrate into the region; proliferate to restore the flawed area; and finally, return to a nonproliferative state. This process involves the regulation of many genes. To elucidate these genes, an in vitro wound healing model using endothelial cell cultures, maintained on an artificial basement membrane of type IV collagen, is being developed. Differential display will be utilized to isolate and identify mRNAs that are regulated during this wound healing process. This approach, along with the use of an in vivo model of infective endocarditis, may provide insights into the disease mechanisms underlying the healing of injured or diseased heart valves. Which may become the site for the development of platelet vegetations. Differential gene regulation during wound healing of this relatively avascular, collagenous valve tissue, may play a key role determining whether repair or vegetation formation occurs. Key words: wound healing, endothelial cell, differential display, infective endocarditis.