SIZE AND DYNAMICS OF MICELLES

时间分辨荧光猝灭(TRFQ)测量将 是在胶束和囊泡系统上进行的，这些系统是模型 以及在药物输送和AS中的应用 生化反应中间TRFQ产生关于大小的信息 胶束分布、添加剂的统计分布和 添加剂间双分子碰撞速率的动力学 胶束。目标是(1)进行TRFQ实验 具体制度；(2)应用TRFQ和TRFQ的组合技术 相同体系的电子顺磁共振(EPR)和 对来自双方的数据达成相互一致的解释 实验；并由此证明使用补充性 技术，极大地提高了信息质量；(4)应用 研究磷脂胶束和囊泡的方法。这个 长期目标是有助于(1)阐明 生物膜结构，(2)基本原理的理解 自组织；(3)多学科协同研究 化学系和生物系的同事们。在TRFQ中， Py将作为荧光探针和无氮氧化合物 作为猝灭剂的自由基(也是顺磁性分子)。在……里面 在解释实验数据时，通常认为添加剂 在胶束中随机分布。在颁奖期内 实验将在一些经过充分研究的系统上进行，例如 在添加剂存在的情况下，使用十二烷基硫酸钠、十六烷基硫酸钠和CTAC。在TRFQ中 方法利用荧光光谱分析技术，对荧光光谱进行实时衰减。 量过了。衰变时间被缩短(淬灭) 由于与探测器的碰撞而产生的猝灭。淬火 速率常数取决于探头之间的碰撞频率 以及胶束内或胶束处的猝灭剂，这又取决于 胶束大小。有一个框架可用来将总体 衰变特性和猝灭速率常数 胶束的平均大小和大小。在互补性中 CER实验将由CSUN的贝尔斯教授在 相同体系下，氮氧自由基的自旋弛豫时间会 量过了。双分子碰撞缩短了关联时间， 碰撞频率取决于胶束大小。提取的 胶束的大小和分布取决于统计上的假设 所有添加剂在胶束上的分布。统计数字 添加剂的分布将进行调整，以符合数据 从这两个实验中。这两种技术一起使用在 因此，相同的系统将给出准确可靠的检测 胶束的大小和添加剂的分布。

Time-resolved fluorescence quenching (TRFQ) measurements will be performed on systems of micelles and vesicles which are models of lipid biomembranes and find applicatins in drug delivery and as biochemical reaction medial TRFQ yields information on the size distribution of micelles, the statistical distribution of additives and the dynamics of bimolecular collision rates between additives on the micelles. The goals are (1) perform TRFQ experiments on specific systems; (2) apply the combined techniques of TRFQ and electron paramagnetic resonance (EPR) on identical systems and arrive at a mutually consistent interpretation of data from both experiments; and thus show that the use of complementary techniques, greatly improves the quality of information; (4) apply the method to the study of phospholipid micelles and vesicles. The long term goal is to contribute to (1) the elucidation of biomembrane structure, (2) the understanding of the fundamentals of self-organization; (3) a synergetic multidisciplinary research with colleagues in the Chemistry and Biology departments. In TRFQ, pyrene will function as the fluorescence probe and a nitroxide free radical as the quencher (also a paramagnetic molecule). In interpreting experimental data, it is usually assumed that additives distribute randomly among the micelles. During the award period experiments will be conducted on some well studied systems, like SDS, LiDS, and CTAC in the presence of additives. In the TRFQ method the real time decay on the pyrene fluorescence is measured. The decay time is shortened (quenched) by the quenchers as a result of collisions with the probe. The quenching rate constant depends on the collision frequency between probe and quencher in or at the micelles, which in turn depends on themicelle size. A framework is available that relates the overall decay characteristics and the quenching rate constant to the average size and size disperson of micelles. In the complementary EPR experiments to be carried out by Prof. Bales at CSUN,on the same systems, spin relaxation time of nitroxide free radicals will be measured. Bimolecular collisions shorten the relatation time and the collision frequency depends on the micelle size. The extracted micelle sizes and distribution rely on assumptions of the statistical distribution of all additives on the micelles. The statistical distribution of the additives will be adjusted to conform to data from both experiments. The two techniques used together on the same system will thus give an accurate and reliable determination of the sizes of micelles and of the distribution of the additives.