ORAL IMPLICATIONS OF HUMAN HERPES VIRUS 8 (HHV8)

描述：人类疱疹病毒8型(HHV-8)已在卡波西病中被发现 肉瘤(KS)以及各种其他组织中的肉瘤，包括 口腔。这项建议旨在确定1)细胞类型 支持HHV-8感染的口腔粘膜，2)细胞依赖性 病毒复制的鉴别，3)口服HHV-8的相关性 口腔KS感染和口腔溃疡，4)HIV-1的作用 混合感染对HHV-8活性的增强和5)评价唾液作为一种 HHV-8变速器的车辆。原位杂交和原位聚合酶链式反应 将使用技术来识别携带HHV-8和HIV-1的细胞 基因组和分化上皮细胞的培养。一个有限的 将对HIV-1阳性患者进行队列和研究，以确定 与口腔KS发展相关的参数。

DESCRIPTION: Human herpes virus 8, (HHV-8) has been identified in Kaposis sarcoma (KS) as well as in a variety of other tissues including those from the oral cavity. This proposal seeks to determine 1) the cell types in the oral mucosa that support HHV-8 infection, 2) the dependency of cellular differentiation on viral replication, 3) the association of oral HHV-8 infection with oral KS and oral ulceration, 4) the role of HIV-1 co-infection in enhancement of HHV-8 activity and 5) evaluate saliva as a vehicle of HHV-8 transmission. In-situ hybridization and in-situ PCR techniques will be used to identify the cells harboring HHV-8 and HIV-1 genomes and in the cultures of differentiating epithelial cells. A limited cohort and study will be done on HIV-1 positive patients to determine parameters associated with the development of oral KS.