HLA-G AND MATERNAL AND FETAL IMMUNITY

描述(改编自申请者的描述)：长期目标 本研究旨在探讨以下假设：可溶性人类白细胞抗原G 从胎盘中提取对母体和胎儿有深远的影响 对半异基因妊娠有利的免疫力。为了探索这一假设 在实验上，研究人员计划开发新的工具和密钥 初步数据。具体目标是：1)准备和表征 用于鉴定特定人类白细胞抗原G的分子探针和抗体 转录本和蛋白质；2)为了：a)建立一个体外哺乳动物 表达系统产生可溶性人类白细胞抗原-G；(B)利用可溶性人类白细胞抗原-G 建立ELISA法；以及c)使用该ELISA法检测来自 3)测定孕妇和非妊娠妇女血清中可溶性人类白细胞抗原-G。 通过确定胎盘是否是可溶性人类白细胞抗原-G的来源 在特定细胞系中的转录和蛋白质生产模式 应用原位杂交技术检测早孕和足月胎盘 免疫组织学。

DESCRIPTION (Adapted from applicant's description): The long-term goal of this research is to investigate the following hypothesis: Soluble HLA-G derived from placentas has a profound influence on maternal and fetal immunity that benefits semiallogeneic pregnancy. To explore this postulate experimentally, the investigator plans to develop new tools and key preliminary data. Specific Aims are: 1) To prepare and characterize molecular probes and antibodies for identification of specific HLA-G transcripts and proteins; 2) To: a) develop an in vitro mammalian expression system to produce soluble HLA-G; (b) use the soluble HLA-G to establish an ELISA assay; and c) use the ELISA assay to test sera from nonpregnant and pregnant women for soluble HLA-G; and 3) To determine whether or not the placenta is a source of soluble HLA-G by ascertaining transcript and protein production patterns in specific cell lineages in first trimester and term placentas using in situ hybridization and immunohistology.