TRYPTOPHAN DEPLETION AND CSF MONOAMINE METABOLITES

血浆色氨酸(Trp)的耗竭越来越多地被许多人使用 研究人员作为确定大脑角色的研究工具 5-羟色胺(5-羟色胺)在多种精神疾病中的作用 它的简单性、安全性和强健的行为效果 一些病人团体。然而，到目前为止还没有研究表明这一点 这项技术实际上改变了人类大脑中的5-羟色胺代谢产物。本研究 将测定脑脊液(CSF)的单胺代谢物 12例缓解性抑郁症患者对色氨酸耗竭的反应 没有症状，至少有3年没有用药 12个月，12名年龄性别匹配的健康对照组。这是意料之中的 缓解后的抑郁受试者会经历抑郁的复发 色氨酸耗竭时的症状，但不是健康对照组。之后 初步筛查、知情同意和基线评估，12 符合DSM-IV标准的患者过去有过严重的抑郁发作， Ham-D得分大于或等于9分，年龄性别匹配12分 没有精神障碍个人或家族病史的受试者 接受测试。将进行色氨酸消耗和对照测试 采用安慰剂对照、双盲、随机、交叉设计。 这包括两次为期三天的测试，每周一次。在一项测试中，受试者 将获得103克，无Trp，15个氨基酸的饮料(Trp消耗)和 在另一项测试中，添加了105克色氨酸的16种氨基酸饮料 (控制)。血浆游离色氨酸和总色氨酸、大分子中性氨基酸 级别和行为评级将在5小时前和5小时之前获得 摄取每一种氨基酸饮料后。这样做的主要目的是 项目是确定色氨酸耗竭是否导致脑脊液5-HIAA的减少 但在HVA和MHPG中不存在，并确定哪种血浆中的色氨酸含量 耗竭与脑脊液5-HIAA的相关性最好。此外，这个项目 将使我们能够获得关于变化关系的初步数据 在色氨酸耗竭至脑脊液5-HIAA期间的情绪变化。这项研究将提供 解释血浆色氨酸相关性的基本数据 人类的5-羟色胺功能衰竭。初步数据显示， 脑脊液5-HIAA与色氨酸耗竭后抑郁反应的关系 作为莫雷诺博士未来调查的基础。

Plasma tryptophan (TRP) depletion is increasingly being used by many investigators as a research tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders because of its simplicity, safety, and robust behavioral effects observed in some patient groups. However no study to date has shown that this technique actually alters brain 5-HT metabolites in humans. This study will determine the cerebrospinal fluid (CSF) monoamine metabolite response to TRP depletion in 12 remitted depressed patients who have been symptom-free, and medication-free for a period of at least 3 months, and 12 age-gender matched healthy controls. It is anticipated that remitted depressed subjects will experience a relapse of depressive symptoms during TRP depletion, but not the healthy controls. After initial screening, informed consent, and baseline assessment, 12 patients meeting DSM-IV criteria for a past major depressive episode, and HAM-D score greater than or equal to 9, and 12 age-gender matched subjects without personal or family history of mental disorders will undergo testing. TRP depletion and control testing will be administered in a placebo-controlled, double-blind, randomized, cross-over design. This includes 2 three-day tests 1 week apart. On one test the subjects will receive 103 gm, TRP-free, 15-amino acid drink (TRP depletion) and on the other test a 105 gm, TRP-supplemented 16-amino acid drink (control). Plasma free and total TRP levels, large neutral amino acid levels, and behavioral ratings will be obtained prior to and five hours after ingestion of each amino acid drink. The primary aim of this project is to determine if TRP depletion causes a decrease of CSF 5-HIAA but not in HVA and MHPG, and to determine which plasma measure of TRP depletion best correlate with CSF 5-HIAA. Additionally this project will allow us to obtain preliminary data on the relationship of change in mood during TRP depletion to CSF 5-HIAA. This study will provide essential data on which to interpret the relevance of plasma TRP depletion to 5-HT function in humans. Preliminary data regarding the relationship of CSF 5-HIAA to depressive response to TRP depletion will serve as a basis for future investigations by Dr. Moreno.