MOLECULAR CLONING OF CARNITINE/ACYLCARNITINE TRANSLOCASE

描述(改编自申请人的描述)：本报告的目的 该项目是对线粒体编码基因的克隆 肉碱/酰基肉碱转位酶。新的一套具体目标包括： 1)人CAC、cDNA3个结构基因的克隆和测序 CAC缺乏症突变基因的测序，3)表达的检测 突变成纤维细胞系中突变基因的选择，4)突变体的产生 在他们认为是酵母CAC的酵母基因yOR100c中进行了鉴定 5)在哺乳动物细胞中检测CAC活性。 已经引进了哪些突变型和野生型CAC。

DESCRIPTION (Adapted from applicant's description): The objective of this project is the cloning of the gene encoding mitochondrial carnitine/acylcarnitine translocase. The new set of Specific Aims include: 1) cloning and sequencing of the human CAC, cDNA and structural gene, 2) sequencing of CAC deficiency mutant genes, 3) examination of the expression of the mutant genes in mutant fibroblast cell lines, 4) creation of mutants in a yeast gene, yOR100c, that they believe to be yeast CAC and assessment of its genetic function, and 5) assaying CAC activity in mammalian cells in which mutant and wild-type CAC has been introduced.